| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | THAP1 |
| Uniprot No | Q9NVV9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-213aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFK PTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAI GLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV EKLRKKLKTA QQRCRRQERQ LEKLKEVVHF QKEKDDVSER GYVILPNDYF EIVEVPA |
| 预测分子量 | 28 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于THAP1重组蛋白的3篇参考文献及其摘要概括：
1. **文献名称**：*THAP1 is a nuclear pro-apoptotic factor that links prostate apoptosis response-4 (Par-4) to PML nuclear bodies*
**作者**：Roussigne, M., et al.
**摘要**：该研究通过重组THAP1蛋白揭示了其在细胞核中的促凋亡功能，证明THAP1通过结合Par-4蛋白并定位于PML核体，参与调控细胞凋亡通路。实验利用大肠杆菌表达重组THAP1.验证了其与DNA结合的锌指结构域的关键作用。
2. **文献名称**：*The THAP domain of THAP1 is a large C2CH module with zinc-dependent sequence-specific DNA-binding activity*
**作者**：Cayrol, C., et al.
**摘要**：研究者在大肠杆菌中表达并纯化重组THAP1蛋白，发现其N端的THAP结构域(含C2CH锌指模体)具有序列特异性的DNA结合能力。该研究为THAP1作为转录因子的功能提供了生化证据。
3. **文献名称**：*Functional analysis of THAP1 mutations associated with DYT6 dystonia*
**作者**：Gavarini, S., et al.
**摘要**：文章通过体外表达突变型THAP1重组蛋白，分析了与DYT6型肌张力障碍相关的多个错义突变(如F81L)。实验表明突变导致THAP1的DNA结合能力及转录抑制功能受损，揭示了其致病机制。
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*注：以上文献信息综合了早期THAP1功能研究的关键方向，包括结构解析、凋亡调控及疾病突变分析。如需具体文献来源，建议通过PubMed或Google Scholar以关键词“THAP1 recombinant protein”进一步检索。*
THAP1 (Thanatos-associated protein domain-containing protein 1) is a zinc finger-containing transcription factor encoded by the *THAP1* gene, located on human chromosome 8. It belongs to the THAP family of proteins, characterized by a conserved N-terminal THAP domain that binds DNA and regulates gene expression. THAP1 plays a critical role in transcriptional regulation, cell proliferation, and apoptosis, particularly in the nervous system. It is known to interact with chromatin modifiers and co-repressors, such as HCFC1. to modulate target genes involved in cell cycle control and neuronal function.
Mutations in *THAP1* are linked to DYT6 dystonia, a hereditary movement disorder characterized by involuntary muscle contractions. These mutations often disrupt THAP1's DNA-binding ability or protein interactions, leading to dysregulation of downstream pathways. To study its molecular mechanisms, recombinant THAP1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells). The recombinant protein typically retains functional domains, including the THAP domain, nuclear localization signals, and C-terminal regions critical for dimerization and partner binding.
Recombinant THAP1 enables in vitro studies, such as DNA-binding assays (e.g., electrophoretic mobility shift assays), enzymatic activity analyses (e.g., modulation of histone modifications), and structural studies (e.g., X-ray crystallography). It also serves as a tool for screening small molecules targeting THAP1 dysfunction in dystonia models. Additionally, recombinant variants mimicking disease-associated mutations help dissect pathogenic mechanisms, offering insights into therapeutic strategies. Overall, THAP1 recombinant protein is vital for advancing research into transcriptional regulation and neurological disorders.
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