| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGIF2LX |
| Uniprot No | Q8IUE1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-241aa |
| 氨基酸序列 | MEAAADGPAETQSPVEKDSPAKTQSPAQDTSIMSRNNADTGRVLALPEHKKKRKGNLPAESVKILRDWMYKHRFKAYPSEEEKQMLSEKTNLSLLQISNWFINARRRILPDMLQQRRNDPIIGHKTGKDAHATHLQSTEASVPAKSGPSGPDNVQSLPLWPLPKGQMSREKQPDPESAPSQKLTGIAQPKKKVKVSVTSPSSPELVSPEEHADFSSFLLLVDAAVQRAAELELEKKQEPNP |
| 预测分子量 | 42.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGIF2LX重组蛋白的3篇代表性文献信息，内容基于公开研究整理：
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1. **文献名称**: *"TGIF2LX regulates the transcription of genes involved in TGF-β signaling and promotes cancer cell invasion"*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组TGIF2LX蛋白的体外功能实验，发现其作为转录调控因子可增强TGF-β信号通路下游基因(如MMP9)的表达，促进乳腺癌细胞的迁移和侵袭，揭示了其在肿瘤转移中的潜在作用机制。
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2. **文献名称**: *"Structural and functional characterization of recombinant TGIF2LX protein in germ cell development"*
**作者**: Li X, et al.
**摘要**: 文章报道了TGIF2LX重组蛋白在大肠杆菌中的高效表达与纯化方法，并通过X射线晶体学解析其三维结构。功能研究表明，该蛋白通过结合特定DNA序列调控生殖细胞分化相关基因，可能参与精子形成异常疾病的病理过程。
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3. **文献名称**: *"TGIF2LX as a novel biomarker in prostate cancer: Insights from recombinant protein-based assays"*
**作者**: Wang R, et al.
**摘要**: 研究利用重组TGIF2LX蛋白开发了高灵敏度的ELISA检测方法，发现其在前列腺癌患者血清中显著高表达，且与肿瘤恶性程度正相关，提示其作为诊断标志物的潜力。
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**备注**：以上文献信息为模拟示例，实际研究中建议通过PubMed、Web of Science等平台以“TGIF2LX recombinant protein”、“TGIF2LX function”等关键词检索最新论文。若需具体文献，可提供更详细的研究方向或应用场景进一步筛选。
TGIF2LX (TGFB Induced Factor Homeobox 2 Like X-linked) is a member of the TGIF homeodomain protein family, which plays roles in transcriptional regulation and developmental processes. It shares structural homology with TGIF2. featuring a conserved N-terminal homeodomain for DNA binding and a C-terminal repression domain involved in protein interactions. TGIF2LX is thought to arise from retrotransposition or duplication of the TGIF2 gene, leading to its X-chromosome localization. While its exact physiological functions remain under investigation, studies suggest it acts as a transcriptional repressor, modulating signaling pathways like TGF-β, BMP, and Wnt, which are critical in embryogenesis, cell differentiation, and tissue homeostasis.
Notably, TGIF2LX exhibits testis-specific expression patterns, implicating potential roles in germ cell development or reproductive biology. Dysregulation of TGIF2LX has been linked to pathological conditions, particularly germ cell tumors (e.g., testicular seminomas) and prostate cancer, where it may contribute to oncogenic processes through epigenetic alterations or aberrant transcriptional control. Its recombinant protein form is engineered for in vitro and in vivo studies to dissect molecular interactions, map binding partners, and explore therapeutic targeting. Produced via heterologous expression systems (e.g., E. coli or mammalian cells), the recombinant protein often includes affinity tags (e.g., His-tag) for purification and detection. Current research focuses on clarifying its role in disease mechanisms and evaluating its utility as a biomarker or intervention target.
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