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产品描述：人核苷酸结合寡聚结构域蛋白1(NOD1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的NOD1含量。NOD1是重要的细胞内模式识别受体，在免疫调节等生理过程起关键作用。其研究机制主要是能识别细菌细胞壁中的特定成分，激活下游信号通路，引发炎症反应，对于理解感染性疾病、自身免疫病发病机制及开发相关疗法有重要意义。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，适用于体外实验中NOD1蛋白表达的定量分析，例如探究病原体感染过程中宿主免疫反应、炎症相关信号通路调控机制或评估药物/化合物对NOD1表达水平的影响。其兼容多种生物样本类型的特点，为细胞模型、动物模型及离体组织样本的研究提供了灵活选择，可广泛应用于免疫学、微生物学及炎症相关疾病的分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：CARD 4 ELISA Kit; CARD4 ELISA Kit; Caspase recruitment domain 4 ELISA Kit; Caspase recruitment domain containing protein 4 ELISA Kit; Caspase recruitment domain family member 4 ELISA Kit; Caspase recruitment domain protein 4 ELISA Kit; Caspase recruitment domain-containing protein 4 ELISA Kit; CLR 7.1 ELISA Kit; CLR7.1 ELISA Kit; NLR family CARD domain containing 1 ELISA Kit; NLRC 1 ELISA Kit; NLRC1 ELISA Kit; NOD 1 ELISA Kit; Nod1 ELISA Kit; NOD1 protein ELISA Kit; NOD1_HUMAN ELISA Kit; Nucleotide binding oligomerization domain containing 1 ELISA Kit; Nucleotide binding oligomerization domain leucine rich repeat and CARD domain containing 1 ELISA Kit; Nucleotide-binding oligomerization domain-containing protein 1 ELISA Kit; Protein Nod1 ELISA Kit
缩写：NOD1
Uniprot No.：Q9Y239
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：15.6 pg/mL-1000 pg/mL
灵敏度：3.9 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cell Biology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human NOD1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 92 | ||||
| Range % | 88-100 | |||||
| 1:2 | Average % | 97 | ||||
| Range % | 92-105 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 82-104 | |||||
| 1:5 | Average % | 96 | ||||
| Range % | 90-102 | |||||
回收率：
| The recovery of human NOD1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 97 | 95-105 | ||||
| EDTA plasma (n=4) | 98 | 95-102 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected10002.2132.0752.1441.9965001.4361.3961.4161.2682500.8240.8160.8200.6721250.4680.4310.4500.30262.50.3690.3510.3600.21231.20.2710.2620.2670.11915.60.2030.1980.2010.05300.1490.1470.148 | ||||||
货期：3-5 working days
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