首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TGFB3 |
| Uniprot No | P10600 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 301-412aa |
| 氨基酸序列 | ALDTNYCFRNLEENCCVRPLYIDFRQDLGWKWVHEPKGYYANFCSGPCPYLRSADTTHSTVLGLYNTLNPEASASPCCVPQDLEPLTILYYVGRTPKVEQLSNMVVKSCKCS |
| 预测分子量 | 16.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TGFB3重组蛋白的3篇示例文献摘要(注：文献为示例性内容，实际引用请核对真实来源)：
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1. **文献名称**：*"Recombinant human TGFB3: High-yield expression in E. coli and functional characterization in vitro"*
**作者**：Smith A, et al.
**摘要**：本研究报道了通过大肠杆菌表达系统高效生产重组人TGFB3蛋白的优化方法，并通过体外实验验证其生物活性，证明其可激活SMAD信号通路并诱导间充质干细胞向软骨细胞分化。
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2. **文献名称**：*"TGFB3-mediated reduction of cardiac fibrosis in a murine myocardial infarction model"*
**作者**：Chen L, et al.
**摘要**：该研究利用重组TGFB3蛋白治疗小鼠心肌梗死模型，发现其通过抑制成纤维细胞过度活化显著减少心脏纤维化，为心脏修复提供了潜在治疗策略。
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3. **文献名称**：*"Differential receptor binding specificity of TGFB3 versus TGFB1 revealed by cryo-EM analysis"*
**作者**：Wang Y, et al.
**摘要**：通过冷冻电镜技术解析TGFB3重组蛋白与细胞表面受体复合物的结构，揭示其与TGFB1不同的受体结合模式，解释了二者在胚胎发育中功能差异的结构基础。
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(如需真实文献，建议在PubMed等数据库以“TGFB3 recombinant protein”、“TGFB3 expression”等关键词检索。)
**Background of Recombinant TGFB3 Protein**
Transforming Growth Factor Beta 3 (TGFB3) is a secreted multifunctional cytokine belonging to the TGF-β superfamily, which regulates diverse cellular processes, including proliferation, differentiation, apoptosis, and extracellular matrix production. It shares structural homology with TGFB1 and TGFB2 but exhibits distinct biological roles, particularly in embryonic development, tissue repair, and immune modulation. TGFB3 is notably critical in palatogenesis, cardiac morphogenesis, and wound healing, where it promotes scarless repair by modulating inflammation and fibroblast activity. Dysregulation of TGFB3 signaling is linked to fibrosis, cancer progression, and developmental disorders.
Recombinant TGFB3 protein is engineered using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to produce high-purity, bioactive forms of the protein for research and therapeutic applications. Unlike endogenous TGFB3. which requires proteolytic activation, recombinant variants are often designed as soluble, mature peptides to ensure consistent activity. Production typically involves codon optimization, affinity tag incorporation (e.g., His-tag), and rigorous purification steps (e.g., chromatography) to eliminate endotoxins and aggregates.
In research, recombinant TGFB3 is widely used to study TGF-β signaling pathways, epithelial-mesenchymal transition (EMT), and tissue regeneration mechanisms. Its therapeutic potential is explored in preclinical models for mitigating fibrosis, accelerating wound healing, and enhancing stem cell-based therapies. Additionally, it serves as a key component in *in vitro* cell culture systems to maintain pluripotency or direct differentiation of stem cells. Despite challenges in balancing its dual roles as both a tumor suppressor and promoter, recombinant TGFB3 remains a vital tool for unraveling developmental biology and advancing regenerative medicine strategies.
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