| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Wnt5b;;Wnt5b |
| WB Predicted band size | Calculated MW: 40 kDa ; Observed MW: 45 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Wnt5b |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Wnt5b抗体的模拟参考文献示例(建议通过PubMed或Google Scholar搜索最新文献)：
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1. **文献名称**：*Wnt5b regulates epithelial-mesenchymal transition in gastric cancer via the non-canonical Wnt pathway*
**作者**：Zhang Y, et al.
**摘要**：本研究利用Wnt5b特异性抗体通过免疫组化和Western blot分析，揭示了Wnt5b在胃癌组织中高表达，并证实其通过激活非经典Wnt信号通路促进肿瘤细胞侵袭和转移。
2. **文献名称**：*Development and validation of a novel monoclonal Wnt5b antibody for immunohistochemical analysis*
**作者**：Smith JL, et al.
**摘要**：文章报道了一种新型Wnt5b单克隆抗体的开发与验证，该抗体在人类组织样本中表现出高特异性，成功应用于乳腺癌和结肠癌的蛋白定位及定量研究。
3. **文献名称**：*Wnt5b signaling in stem cell differentiation: Insights from antibody-mediated inhibition*
**作者**：Chen R, et al.
**摘要**：通过使用Wnt5b中和抗体，研究团队发现Wnt5b在间充质干细胞成骨分化中的关键作用，表明其通过调节JNK信号通路影响细胞命运。
4. **文献名称**：*Dysregulation of Wnt5b in obesity-associated adipose tissue inflammation*
**作者**：Kim H, et al.
**摘要**：利用Wnt5b抗体进行组织染色和流式细胞术，研究发现肥胖小鼠脂肪组织中Wnt5b表达显著上调，并与巨噬细胞浸润及慢性炎症相关。
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**建议**：如需真实文献，可通过以下关键词检索：
- 数据库：PubMed、Web of Science
- 关键词：`Wnt5b antibody`、`Wnt5b immunohistochemistry`、`Wnt5b signaling`
- 筛选条件：优先选择近5年发表且高被引的研究。
**Background of Wnt5b Antibody**
The Wnt5b antibody is a specialized tool used to detect and study Wnt5b, a member of the Wnt protein family critical for regulating developmental processes and cellular signaling. Wnt5b, a secreted glycoprotein, belongs to the non-canonical Wnt signaling pathway, which influences cell migration, polarity, and tissue organization. It shares structural and functional similarities with Wnt5a but exhibits distinct expression patterns, particularly in embryonic development, adult tissues (e.g., adipose, kidney), and pathological contexts like cancer. Dysregulation of Wnt5b is linked to metabolic disorders, fibrosis, and tumor progression, where it may promote invasiveness or modulate microenvironment interactions.
Wnt5b antibodies are designed to recognize specific epitopes on the Wnt5b protein, enabling applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). These antibodies help researchers assess Wnt5b expression levels, localization, and activity in biological samples. Polyclonal antibodies offer broad epitope recognition, while monoclonal antibodies provide high specificity. Validation steps (e.g., knockout controls, peptide blocking) ensure reliability in experimental models.
As Wnt signaling research expands, Wnt5b antibodies remain vital for exploring its role in development, disease mechanisms, and potential therapeutic targeting. Their utility spans cancer biology, stem cell research, and metabolic studies, highlighting their importance in both basic and translational science.
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