| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEB1 |
| Uniprot No | Q15369 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-112aa |
| 氨基酸序列 | MGSSHHHHHHSSGLVPRGSHMDGEEKTYGGCEGPDAMYVKLISSDGHEFI VKREHALTSGTIKAMLSGPGQFAENETNEVNFREIPSHVLSKVCMYFTYK VRYTNSSTEIPEFPIAPEIALELLMAANFLDC |
| 预测分子量 | 15 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCEB1重组蛋白的3条参考文献的简要列举(模拟内容，非真实文献)：
1. **文献名称**：Structural insights into the Elongin-C complex: TCEB1 as a key component in transcriptional regulation
**作者**：Li, X., et al.
**摘要**：本研究通过重组表达纯化人源TCEB1蛋白，结合晶体学分析揭示了其与Elongin B/C复合物的相互作用机制，阐明了TCEB1在RNA聚合酶II转录延伸中的结构基础。
2. **文献名称**：Recombinant TCEB1 facilitates HIF-1α degradation via VHL ubiquitin ligase assembly
**作者**：Yamada, K., et al.
**摘要**：文章报道了重组TCEB1蛋白在体外促进Von Hippel-Lindau(VHL)肿瘤抑制蛋白与Elongin C/B复合物组装的作用，证明其通过调控HIF-1α泛素化降解途径参与缺氧信号传导。
3. **文献名称**：Functional characterization of TCEB1 mutations in renal cell carcinoma using recombinant protein models
**作者**：Zhang, R., et al.
**摘要**：通过构建携带肾癌相关突变的TCEB1重组蛋白，研究者发现特定突变会破坏其与Cul2-RING E3连接酶的相互作用，导致HIF通路异常激活，为靶向治疗提供实验依据。
注：以上内容为示例性模拟，实际文献需通过PubMed/Google Scholar等平台检索确认。
**Background of TCEB1 Recombinant Protein**
TCEB1 (Transcription Elongation Factor B Polypeptide 1), also known as Elongin B, is a critical subunit of the Elongin complex, which includes Elongin A (transcriptional activator), Elongin B, and Elongin C. This evolutionarily conserved complex plays a dual role in transcriptional elongation and protein ubiquitination. TCEB1. encoded by the *TCEB1* gene, stabilizes the interaction between Elongin A and Elongin C, facilitating RNA polymerase II-mediated transcription elongation by suppressing transient pausing. Beyond transcription, TCEB1 is integral to the von Hippel-Lindau (VHL) tumor suppressor complex, where it partners with VHL protein and Elongin C to form an E3 ubiquitin ligase. This complex targets hypoxia-inducible factors (HIFs) for proteasomal degradation under normoxic conditions, a process dysregulated in cancers and VHL syndrome.
Recombinant TCEB1 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional protein for biochemical and cellular studies. It typically retains post-translational modification sites and structural motifs necessary for binding Elongin C and participating in multiprotein assemblies. Researchers utilize TCEB1 recombinant protein to explore its role in transcription regulation, oxygen-sensing pathways, and ubiquitination cascades. It also serves as a tool for structural studies (e.g., crystallography) and screening therapeutic agents targeting VHL-associated diseases or cancers linked to HIF dysregulation. Its high purity and activity make it invaluable for dissecting molecular mechanisms underlying both normal cellular processes and pathologies.
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