首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔子髓过氧化物酶(MPO)酶联免疫试剂盒为竞争法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MPO含量。MPO即髓过氧化物酶，是中性粒细胞和单核细胞产生的一种血红素蛋白。其可催化过氧化氢和氯离子生成次氯酸，在固有免疫中发挥重要作用。异常表达的MPO会产生大量活性氧，与多种炎症相关疾病和心血管疾病的发病机制有关。试剂盒检测范围为1.25 ng/mL-20 ng/mL，适用于基础科研中炎症机制研究、免疫相关疾病动物模型评估、药物干预效果分析等场景，例如通过检测实验动物血清或组织样本中的MPO水平，定量评估中性粒细胞浸润程度及相关疾病的病理进程，为心血管疾病、神经退行性疾病等领域的机制探索提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：MPO
Uniprot No.：G1SR65
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：1.25 ng/mL-20 ng/mL
灵敏度：1.25 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Competitive
精密度：
| Intra-assay Precision (Precision within an assay): CV%<15% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<15% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MPO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 96 | ||||
| Range % | 91-103 | |||||
| 1:2 | Average % | 87 | ||||
| Range % | 84-90 | |||||
| 1:4 | Average % | 95 | ||||
| Range % | 89-99 | |||||
| 1:8 | Average % | 101 | ||||
| Range % | 98-105 | |||||
回收率：
| The recovery of rabbit MPO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 89 | 85-94 | ||||
| EDTA plasma (n=4) | 93 | 89-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2Average 200.2040.2010.203 100.3760.3980.387 50.6350.6020.619 2.51.1341.1221.128 1.252.3392.3672.353 | ||||||
货期：3-5 working days
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