首页 / 产品 / ELISA试剂盒 / 人ELISA试剂盒
产品描述：人基质金属蛋白酶-20(MMP20)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MMP20含量。MMP20 即基质金属蛋白酶 20，在牙釉质发育中起关键作用。其研究机制主要聚焦于参与牙釉质蛋白水解、调控釉质矿化过程等方面。对它的深入研究有助于理解牙釉质形成机理，为牙釉质发育异常相关疾病的诊治提供思路。试剂盒检测范围为0.312 ng/mL-20 ng/mL，牙齿发育相关疾病机制研究、口腔组织工程及病理过程分析、牙釉质发育调控、口腔疾病模型构建、生物材料降解评估、细胞生物学、组织工程及疾病机制等领域的科研需求本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：AI2A2 ELISA Kit; Enamel metalloproteinase ELISA Kit; Enamelysin ELISA Kit; Matrix metalloproteinase 20 ELISA Kit; Matrix metalloproteinase-20 ELISA Kit; MMP 20 ELISA Kit; MMP-20 ELISA Kit; MMP20 ELISA Kit; MMP20_HUMAN ELISA Kit
缩写：MMP20
Uniprot No.：O60882
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.312 ng/mL-20 ng/mL
灵敏度：0.078 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | |||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | |||||||
| Inter-assay Precision (Precision between assays): CV%<10% | |||||||
| Three samples of known concentration were tested in twenty assays to assess. | |||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP20 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | |||||||
| Sample | Serum(n=4) | ||||||
| 1:1 | Average % | 89 | |||||
| Range % | 85-93 | ||||||
| 1:2 | Average % | 100 | |||||
| Range % | 95-105 | ||||||
| 1:4 | Average % | 86 | |||||
| Range % | 83-89 | ||||||
| 1:8 | Average % | 97 | |||||
| Range % | 92-101 | ||||||
回收率：
| The recovery of human MMP20 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | |||||||
| Sample Type | Average % Recovery | Range | |||||
| Serum (n=5) | 88 | 86-90 | |||||
| EDTA plasma (n=4) | 101 | 97-104 | |||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
| ng/mlOD1OD2AverageCorrected202.3032.2042.2542.101101.9151.9641.9401.78751.6361.5301.5831.4302.51.2011.3011.2511.0981.250.8210.8350.8280.6750.6250.5120.5010.5070.3540.3120.3520.3680.3600.20700.1540.1520.153 | |||||||
货期：3-5 working days
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