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产品描述：人基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的MMP2含量。MMP2即基质金属蛋白酶2，在细胞外基质重塑等生理过程中发挥关键作用。其异常表达与肿瘤侵袭、转移及心血管疾病等密切相关。研究机制主要聚焦于它对细胞外基质降解、信号通路激活等方面，以探寻其在疾病发生发展中的作用及潜在治疗靶点。试剂盒检测范围为0.78 ng/mL-50 ng/mL，其标准化操作流程适用于体外研究场景，例如通过检测细胞培养上清液评估特定刺激下MMP - 2分泌水平变化，或分析组织样本中该酶的表达特征，为疾病分子机制研究、药物作用效果验证及生物标志物筛选提供技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：72 kDa gelatinase ELISA Kit; 72kD type IV collagenase ELISA Kit; CLG 4 ELISA Kit; CLG 4A ELISA Kit; CLG4 ELISA Kit; CLG4A ELISA Kit; Collagenase Type 4 alpha ELISA Kit; Collagenase type IV A ELISA Kit; Gelatinase A ELISA Kit; Gelatinase alpha ELISA Kit; Gelatinase neutrophil ELISA Kit; Matrix metallopeptidase 2 gelatinase A 72kDa gelatinase 72kDa type IV collagenase ELISA Kit; Matrix metalloproteinase 2 (gelatinase A, 72kDa gelatinase, 72kDa type IV collagenase) ELISA Kit; Matrix Metalloproteinase 2 ELISA Kit; Matrix metalloproteinase II ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP 2 ELISA Kit; MMP II ELISA Kit; MMP-2 ELISA Kit; MMP2 ELISA Kit; MMP2_HUMAN ELISA Kit; MONA ELISA Kit; Neutrophil gelatinase ELISA Kit; PEX ELISA Kit; TBE 1 ELISA Kit; TBE-1 ELISA Kit
缩写：MMP2
Uniprot No.：P08253
种属：Homo sapiens (Human)
样本类型：serum, plasma, cell culture supernates, tissue homogenates
检测范围：0.78 ng/mL-50 ng/mL
灵敏度：0.195 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 88 | ||||
| Range % | 80-92 | |||||
| 1:2 | Average % | 99 | ||||
| Range % | 91-107 | |||||
| 1:4 | Average % | 96 | ||||
| Range % | 92-101 | |||||
| 1:8 | Average % | 93 | ||||
| Range % | 86-112 | |||||
回收率：
| The recovery of human MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 88 | 80-98 | ||||
| EDTA plasma (n=4) | 94 | 90-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected502.6592.5882.6242.435252.2102.0802.1451.95612.51.5401.5801.5601.3716.251.0211.0091.0150.8263.120.5880.6200.6040.4151.560.3900.4200.4050.2160.780.2500.2600.2550.06600.1900.1880.189 | ||||||
本试剂盒所含材料：
A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human MMP-2 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
One vial Biotin-labeled MMP-2 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
本试剂盒不含材料：
A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
An incubator can provide stable incubation conditions up to 37°C±5°C.
Centrifuge
Vortex
Squirt bottle, manifold dispenser, or automated microplate washer
Absorbent paper for blotting the microtiter plate
货期：3-5 working days
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