首页 / 产品 / ELISA试剂盒 / 兔ELISA试剂盒
产品描述：兔基质金属蛋白酶2/明胶酶A(MMP-2/Gelatinase A)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MMP2含量。MMP2 即基质金属蛋白酶 2，在多种生理和病理过程中起关键作用，如组织重塑、肿瘤转移等。其研究机制主要围绕它对细胞外基质的降解功能，可破坏基底膜促使肿瘤细胞迁移。深入研究 MMP2 有助于揭示疾病发生发展机制，为相关疾病治疗提供靶点。试剂盒检测范围为1.56 ng/mL-100 ng/mL，支持科研领域对动物疾病模型（如关节炎、肿瘤移植模型）的分子机制研究，或用于细胞培养上清、组织提取液中MMP - 2动态变化的分析，为探索基质代谢调控、药物干预效果评估提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MMP2 ELISA Kit; 72 kDa type IV collagenase ELISA Kit; EC 3.4.24.24 ELISA Kit; 72 kDa gelatinase ELISA Kit; Gelatinase A ELISA Kit; Matrix metalloproteinase-2 ELISA Kit; MMP-2) [Cleaved into: PEX] ELISA Kit
缩写：MMP2
Uniprot No.：P50757
种属：Oryctolagus cuniculus (Rabbit)
样本类型：serum, plasma, tissue homogenates
检测范围：1.56 ng/mL-100 ng/mL
灵敏度：0.39 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rabbit MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:10 | Average % | 89 | ||||
| Range % | 85-95 | |||||
| 1:20 | Average % | 96 | ||||
| Range % | 90-105 | |||||
| 1:40 | Average % | 93 | ||||
| Range % | 85-99 | |||||
| 1:80 | Average % | 94 | ||||
| Range % | 86-98 | |||||
回收率：
| The recovery of rabbit MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 89-103 | ||||
| EDTA plasma (n=4) | 92 | 87-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected1002.5062.5312.5192.419502.0482.0052.0271.927251.6741.6911.6831.58312.51.1841.2071.1961.0966.250.8820.8790.8810.7813.120.5540.5430.5490.4491.560.3060.3140.3100.21000.1020.0970.100 | ||||||
货期：3-5 working days
×
