| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TCEA1 |
| Uniprot No | P23193 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-301aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TCEA1重组蛋白的参考文献示例(注：部分文献为假设性描述，实际研究中请通过学术数据库核实)：
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1. **标题**: "Recombinant Human TCEA1 Protein Enhances Transcriptional Elongation in Vitro"
**作者**: Zhang Y, et al.
**摘要**: 本研究通过在大肠杆菌中表达并纯化重组人TCEA1蛋白，验证其作为转录延伸因子(TFIIS)的功能。实验表明，重组TCEA1可促进RNA聚合酶II在体外转录过程中的停滞恢复，并增强基因表达效率。
2. **标题**: "Structural Analysis of TCEA1 Reveals Critical Domains for DNA Binding"
**作者**: Kimura T, et al.
**摘要**: 通过X射线晶体学解析重组TCEA1蛋白的三维结构，发现其N端结构域对DNA结合和转录延伸调控至关重要，为理解TCEA1在基因调控中的作用提供结构基础。
3. **标题**: "TCEA1 Recombinant Protein Suppresses Tumor Cell Proliferation via p53 Activation"
**作者**: Chen L, et al.
**摘要**: 在癌细胞系中过表达重组TCEA1蛋白，发现其通过激活p53通路抑制细胞增殖，提示TCEA1可能作为潜在肿瘤治疗靶点。
4. **标题**: "High-Yield Expression and Purification of TCEA1 in Insect Cells"
**作者**: Müller S, et al.
**摘要**: 开发了一种基于杆状病毒-昆虫细胞系统的TCEA1重组蛋白高效表达与纯化方法，获得的蛋白适用于功能性研究和药物筛选。
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**建议**：实际文献需通过PubMed、Web of Science等平台检索关键词“TCEA1 recombinant protein”或“TFIIS expression”，并筛选与重组表达、功能验证相关的研究。
**Background of TCEA1 Recombinant Protein**
TCEA1 (Transcription Elongation Factor A1), also known as TFIIS, is a critical regulatory protein involved in transcription elongation by RNA polymerase II (Pol II). It plays a pivotal role in resolving transcriptional pauses or arrests by stimulating the intrinsic nuclease activity of Pol II, allowing the enzyme to cleave backtracked RNA transcripts and resume RNA synthesis. This function is essential for maintaining transcriptional fidelity and efficiency, particularly in genes with complex regulatory regions or repetitive sequences.
Structurally, TCEA1 contains conserved domains, including a N-terminal zinc-binding domain and a C-terminal domain that interacts directly with Pol II. Its ability to bind the elongating polymerase and modulate transcriptional dynamics makes it a key subject in studies of gene expression regulation, epigenetic modifications, and transcriptional stress responses. Dysregulation of TCEA1 has been implicated in various diseases, including cancers, where aberrant transcription elongation contributes to oncogenic gene activation.
Recombinant TCEA1 protein is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells*) to ensure high purity and functionality for experimental applications. It is widely utilized in *in vitro* transcription assays, structural studies (e.g., crystallography or cryo-EM), and drug discovery research targeting transcription-related disorders. The recombinant form often includes affinity tags (e.g., His-tag) for simplified purification and detection.
Current research focuses on elucidating TCEA1’s role in cellular differentiation, stress adaptation, and its interplay with other elongation factors (e.g., P-TEFb) or chromatin remodelers. Understanding TCEA1 mechanisms may unveil therapeutic strategies for diseases linked to transcriptional dysregulation.
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