首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：人甘露聚糖结合凝集素丝氨酸肽1（C4/C2 RA反应因子的激活因子）(MASP1)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MASP1含量。MASP1（甘露聚糖结合凝集素相关丝氨酸蛋白酶 1）在补体系统激活中发挥重要作用。作为补体凝集素途径的关键酶。研究机制上，它能酶解相关底物，启动补体级联反应，与免疫防御、炎症反应等生理病理过程密切相关。试剂盒检测范围为3.12 ng/mL-200 ng/mL，本产品可广泛用于免疫学、炎症性疾病机制研究及药物开发中MASP1表达水平的动态监测，适用于科研领域对补体系统相关生物标志物的定量分析需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：MASP1 ELISA Kit; CRARF ELISA Kit; CRARF1 ELISA Kit; PRSS5Mannan-binding lectin serine protease 1 ELISA Kit; EC 3.4.21.- ELISA Kit; Complement factor MASP-3 ELISA Kit; Complement-activating component of Ra-reactive factor ELISA Kit; Mannose-binding lectin-associated serine protease 1 ELISA Kit; MASP-1 ELISA Kit; Mannose-binding protein-associated serine protease ELISA Kit; Ra-reactive factor serine protease p100 ELISA Kit; RaRF ELISA Kit; Serine protease 5) [Cleaved into: Mannan-binding lectin serine protease 1 heavy chain; Mannan-binding lectin serine protease 1 light chain] ELISA Kit
缩写：MASP1
Uniprot No.：P48740
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：3.12 ng/mL-200 ng/mL
灵敏度：0.78 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Immunology
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MASP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:100 | Average % | 96 | ||||
| Range % | 91-99 | |||||
| 1:200 | Average % | 100 | ||||
| Range % | 95-104 | |||||
| 1:400 | Average % | 94 | ||||
| Range % | 89-98 | |||||
| 1:800 | Average % | 99 | ||||
| Range % | 93-103 | |||||
回收率：
| The recovery of human MASP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 94 | 88-97 | ||||
| EDTA plasma (n=4) | 94 | 90-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected2002.7972.6462.7222.6161002.0262.0002.0131.907501.2551.2441.2501.144250.7010.6980.7000.59412.50.4160.4030.4100.3046.250.3020.2970.3000.1943.120.1870.1960.1920.08600.1080.1040.106 | ||||||
货期：3-5 working days
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