首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠微管相关蛋白2(MAP-2)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆、细胞裂解物样本中的MAP2含量。MAP2，即微管相关蛋白2，是一种在神经元中含量丰富的微管结合蛋白，参与微管组装和稳定，对维持神经元形态和功能至关重要。其研究机制涉及MAP2与微管蛋白的相互作用，以及其在神经元发育和神经退行性疾病中的作用。试剂盒检测范围为0.156 ng/mL-10 ng/mL，该产品适用于神经生物学领域的基础研究，如脑损伤模型、神经退行性疾病机制探索或药物干预实验中MAP - 2的动态变化分析，支持多种样本类型的平行检测，为科研人员提供稳定可靠的数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Map2 ELISA Kit; Mtap2 ELISA Kit; Microtubule-associated protein 2 ELISA Kit; MAP-2 ELISA Kit
缩写：MAP2
Uniprot No.：P15146
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, tissue homogenates, cell lysates
检测范围：0.156 ng/mL-10 ng/mL
灵敏度：0.039 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat MAP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 90 | ||||
| Range % | 86-93 | |||||
| 1:2 | Average % | 103 | ||||
| Range % | 98-105 | |||||
| 1:4 | Average % | 93 | ||||
| Range % | 88-97 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of rat MAP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 95 | 91-98 | ||||
| EDTA plasma (n=4) | 97 | 92-99 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected102.3292.3122.3212.18551.8441.7761.8101.6742.51.2741.2961.2851.1491.250.9230.9170.9200.7840.6250.6340.6240.6290.4930.3120.4330.4430.4380.3020.1560.2970.2820.2900.15400.1380.1340.136 | ||||||
货期：3-5 working days
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