首页 / 产品 / ELISA试剂盒 / 大鼠ELISA试剂盒
产品描述：大鼠Tau蛋白酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、脑脊液（CSF）样本中的MAPT含量。MAPT基因编码tau蛋白，是神经退行性疾病如额颞叶痴呆（FTDP-17）、阿尔茨海默病（AD）等的关键基因。其突变导致tau蛋白与微管结合能力下降，引发神经纤维缠结，进而导致神经元损伤。研究MAPT基因突变与tau蛋白功能异常的机制，有助于开发针对神经退行性疾病的诊断和治疗策略。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于动态监测实验动物模型中Tau蛋白的表达变化；适用于神经系统疾病的基础研究、药物干预效果评估或病理进程分析；为神经生物学、药理学及疾病机制探索提供高效工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Mapt ELISA Kit; Mtapt ELISA Kit; Tau ELISA Kit; Microtubule-associated protein tau ELISA Kit; Neurofibrillary tangle protein ELISA Kit; Paired helical filament-tau ELISA Kit; PHF-tau ELISA Kit
缩写：MAPT
Uniprot No.：P19332
种属：Rattus norvegicus (Rat)
样本类型：serum, plasma, cerebrospinal fluid (CSF)
检测范围：62.5 pg/mL-4000 pg/mL
灵敏度：15.6 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Signal Transduction
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of rat tau proteins in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:5 | Average % | 98 | ||||
| Range % | 95-101 | |||||
| 1:10 | Average % | 90 | ||||
| Range % | 88-92 | |||||
| 1:20 | Average % | 85 | ||||
| Range % | 81-89 | |||||
| 1:40 | Average % | 98 | ||||
| Range % | 95-101 | |||||
回收率：
| The recovery of rat tau proteins spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 101 | 99-105 | ||||
| EDTA plasma (n=4) | 87 | 84-90 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected40002.6942.6782.6862.52320002.1042.1562.1301.96710001.3301.3561.3431.1805000.8250.8360.8310.6682500.5520.5680.5600.3971250.3590.3680.3640.20162.50.2760.2980.2870.12400.1670.1590.163 | ||||||
货期：3-5 working days
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