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产品描述：人黑色素瘤相关抗原3(MAGE-3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、尿液、组织匀浆样本中的MAGEA3含量。MAGEA3是一种肿瘤相关抗原，主要在多种肿瘤组织中表达，具有免疫原性，是细胞免疫治疗的重要靶点。其表达与肿瘤的发生、发展及预后密切相关，可用于肿瘤的诊断和免疫治疗。MAGEA3通过影响抑癌基因活性、调节细胞周期、诱导免疫应答等机制，在肿瘤细胞中发挥重要作用。试剂盒检测范围为0.625 ng/mL-40 ng/mL，适用于肿瘤发生机制研究、抗肿瘤药物疗效评估、肿瘤微环境分析等科研领域，为探索MAGE - 3在肿瘤免疫逃逸中的作用及开发靶向治疗方案提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：Antigen MZ2 D ELISA Kit; Antigen MZ2-D ELISA Kit; Cancer/testis antigen 1.3 ELISA Kit; Cancer/testis antigen family 1 member 3 ELISA Kit; CT1.3 ELISA Kit; HIP8 ELISA Kit; HYPD ELISA Kit; MAGA3_HUMAN ELISA Kit; MAGE 3 antigen ELISA Kit; MAGE family member A3 ELISA Kit; MAGE-3 antigen ELISA Kit; MAGE3 ELISA Kit; MAGEA3 ELISA Kit; MAGEA6 ELISA Kit; Melanoma antigen family A 3 ELISA Kit; Melanoma associated antigen 3 ELISA Kit; Melanoma-associated antigen 3 ELISA Kit; MGC1461 ELISA Kit
缩写：MAGEA3
Uniprot No.：P43357
种属：Homo sapiens (Human)
样本类型：serum, plasma, urine, tissue homogenates
检测范围：0.625 ng/mL-40 ng/mL
灵敏度：0.156 ng/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human MAGE-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 97 | ||||
| Range % | 89-101 | |||||
| 1:2 | Average % | 95 | ||||
| Range % | 87-99 | |||||
| 1:4 | Average % | 88 | ||||
| Range % | 82-93 | |||||
| 1:8 | Average % | 97 | ||||
| Range % | 91-101 | |||||
回收率：
| The recovery of human MAGE-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 104 | 92-107 | ||||
| EDTA plasma (n=4) | 96 | 88-100 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| ng/mlOD1OD2AverageCorrected401.7421.7771.7601.642201.3831.4111.3971.279100.8600.9070.8840.76650.5390.5590.5490.4312.50.3530.3440.3490.2311.250.2190.2220.2210.1030.6250.1750.1810.1780.06000.1170.1190.118 | ||||||
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