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产品描述：人Ly6/PLAUR结构域蛋白3(LYPD3)酶联免疫试剂盒为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LYPD3含量。试剂盒检测范围为31.25 pg/mL-2000 pg/mL，本试剂盒适用于肿瘤微环境研究、细胞间相互作用机制探索或药物开发中生物标志物动态监测等科研场景。满足基础研究与转化医学中对LYPD3蛋白定量分析的需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：2310061G07Rik ELISA Kit; C4.4A ELISA Kit; GPI-anchored metastasis-associated protein C4.4A homolog ELISA Kit; GPI-anchored metastasis-associated protein homolog ELISA Kit; Ly6/PLAUR domain-containing protein 3 ELISA Kit; Lypd3 ELISA Kit; LYPD3_HUMAN ELISA Kit; Matrigel-induced gene C4 protein ELISA Kit; MIG-C4 ELISA Kit; SHINC3 ELISA Kit
缩写：LYPD3
Uniprot No.：O95274
种属：Homo sapiens (Human)
样本类型：serum, plasma, tissue homogenates
检测范围：31.25 pg/mL-2000 pg/mL
灵敏度：7.81 pg/mL
反应时间：1-5h
样本体积：50-100ul
检测波长：450 nm
研究领域：Cancer
测定原理：quantitative
测定方法：Sandwich
精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
| Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
| Inter-assay Precision (Precision between assays): CV%<10% | ||||||
| Three samples of known concentration were tested in twenty assays to assess. | ||||||
线性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | |||||
| 1:1 | Average % | 101 | ||||
| Range % | 94-105 | |||||
| 1:2 | Average % | 88 | ||||
| Range % | 81-93 | |||||
| 1:4 | Average % | 94 | ||||
| Range % | 87-97 | |||||
| 1:8 | Average % | 92 | ||||
| Range % | 84-96 | |||||
回收率：
| The recovery of human LYPD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
| Sample Type | Average % Recovery | Range | ||||
| Serum (n=5) | 90 | 87-94 | ||||
| EDTA plasma (n=4) | 92 | 85-97 | ||||
标准曲线：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | ||||||
| pg/mlOD1OD2AverageCorrected20002.6262.4462.5362.37810002.2282.1132.1712.0135001.5061.4881.4971.3392500.8030.7850.7940.6361250.5020.4910.4970.33962.50.3770.3640.3710.21331.250.2460.2510.2490.09100.1560.1600.158 | ||||||
货期：3-5 working days
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