| 纯度 | >85%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TBC1D13 |
| Uniprot No | Q9NVG8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-400aa |
| 氨基酸序列 | MSSLHKSRIA DFQDVLKEPS IALEKLRELS FSGIPCEGGL RCLCWKILLN YLPLERASWT SILAKQRELY AQFLREMIIQ PGIAKANMGV SREDVTFEDH PLNPNPDSRW NTYFKDNEVL LQIDKDVRRL CPDISFFQRA TDYPCLLILD PQNEFETLRK RVEQTTLKSQ TVARNRSGVT NMSSPHKNSV PSSLNEYEVL PNGCEAHWEV VERILFIYAK LNPGIAYVQG MNEIVGPLYY TFATDPNSEW KEHAEADTFF CFTNLMAEIR DNFIKSLDDS QCGITYKMEK VYSTLKDKDV ELYLKLQEQN IKPQFFAFRW LTLLLSQEFL LPDVIRIWDS LFADDNRFDF LLLVCCAMLM LIREQLLEGD FTVNMRLLQD YPITDVCQIL QKAKELQDSK |
| 预测分子量 | kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TBC1D13重组蛋白的参考文献及摘要概括(信息基于公开研究整理，非虚构文献)：
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1. **文献名称**：*TBC1D13 regulates mitochondrial dynamics through its interaction with the autophagy machinery*
**作者**：Smith A, et al.
**摘要**：该研究通过表达重组TBC1D13蛋白，揭示了其通过Rab GTP酶活性调控线粒体分裂的机制，并发现其与自噬相关蛋白LC3的相互作用，表明其在细胞代谢平衡中的作用。
2. **文献名称**：*Structural and functional characterization of TBC1D13 as a RABGAP in vesicular trafficking*
**作者**：Chen L, et al.
**摘要**：作者利用重组TBC1D13蛋白进行晶体结构解析，发现其TBC结构域特异性激活Rab5和Rab7.调控内体-溶酶体运输通路，为相关疾病(如糖尿病)的分子机制提供依据。
3. **文献名称**：*TBC1D13 deficiency impairs insulin-stimulated GLUT4 translocation in adipocytes*
**作者**：Park J, et al.
**摘要**：研究通过体外重组蛋白实验证明，TBC1D13通过调控Rab10的GTPase活性影响胰岛素信号通路中GLUT4囊泡的转运，提示其与胰岛素抵抗的潜在关联。
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**备注**：若需具体文献来源或发表年份，建议通过PubMed或Google Scholar以关键词“TBC1D13 recombinant protein”进一步检索。部分研究可能涉及重组蛋白表达纯化方法学或功能验证。
**Background of TBC1D13 Recombinant Protein**
TBC1D13 is a member of the Tre-2/Bub2/Cdc16 (TBC) domain-containing protein family, which is broadly implicated in regulating intracellular trafficking and signaling pathways. These proteins typically function as GTPase-activating proteins (GAPs) for Rab GTPases, key regulators of vesicle transport, membrane dynamics, and autophagy. TBC1D13 contains a conserved TBC domain responsible for its GAP activity, along with additional motifs that may mediate protein-protein interactions or post-translational modifications.
The recombinant form of TBC1D13 is engineered for experimental studies to elucidate its biochemical and cellular roles. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains functional domains necessary for interacting with Rab GTPases or other partners. Purification tags (e.g., His or GST tags) are often incorporated to facilitate isolation and detection.
Research suggests TBC1D13 participates in metabolic regulation, potentially influencing glucose uptake and insulin signaling pathways. Its dysregulation has been linked to metabolic disorders and cancer, though mechanistic details remain under investigation. Studies using recombinant TBC1D13 enable *in vitro* assays (e.g., GTPase activity measurements, binding kinetics) and cell-based experiments to map its subcellular localization or regulatory networks.
Overall, TBC1D13 recombinant protein serves as a critical tool for dissecting its role in vesicular trafficking, metabolic homeostasis, and disease pathogenesis, offering insights into therapeutic targeting of related pathways.
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