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Rabbit Monoclonal ABHD5 Antibody

  • 中文名： ABHD5抗体
  • 别    名： ABHD5; Abhydrolase domain containing 5; CDS; CGI58; IECN2; NCIE2;;ABHD5
货号: IPDX18643
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 咨询技术 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesABHD5; Abhydrolase domain containing 5; CDS; CGI58; IECN2; NCIE2;;ABHD5
WB Predicted band sizeCalculated MW: 39 kDa ; Observed MW: 45 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human ABHD5
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3篇关于ABHD5抗体的参考文献，涵盖其功能研究与疾病关联：

1. **文献名称**：*Adipose triglyceride lipase-mediated lipolysis of cellular fat stores is activated by CGI-58 and defective in Chanarin-Dorfman Syndrome*

**作者**：Lass, A., Zimmermann, R., Oberer, M., & Zechner, R. (2006)

**摘要**：研究揭示了ABHD5(CGI-58)通过激活ATGL(脂肪甘油三酯脂肪酶)促进脂解的作用，并发现Chanarin-Dorfman综合征患者中ABHD5功能缺失导致脂质代谢异常。文中使用ABHD5抗体进行Western blot及免疫共沉淀实验验证蛋白相互作用。

2. **文献名称**：*Mutations in CGI-58. the gene encoding a new protein of the esterase/lipase/thioesterase subfamily, in Chanarin-Dorfman syndrome*

**作者**：Lefèvre, C., Jobard, F., Caux, F., et al. (2001)

**摘要**：首次报道了ABHD5(CGI-58)基因突变导致Chanarin-Dorfman综合征，该疾病表现为中性脂质沉积。研究利用ABHD5抗体进行免疫荧光染色，显示突变体在细胞内的定位异常及脂滴积累。

3. **文献名称**：*Growth retardation, impaired triacylglycerol catabolism, hepatic steatosis, and lethal skin barrier defect in mice lacking comparative gene identification-58 (CGI-58)*

**作者**：Radner, F.P., Streith, I.E., Schoiswohl, G., et al. (2010)

**摘要**：通过构建ABHD5基因敲除小鼠模型，发现其皮肤屏障功能障碍及肝脏脂肪变性。研究采用ABHD5抗体进行免疫组化分析，证实ABHD5在小鼠表皮中的特异性表达及其在脂质代谢中的关键作用。

这些文献均通过ABHD5抗体技术手段，深入探讨了该蛋白在脂代谢调控、疾病机制及生理功能中的多方面作用。

背景信息

ABHD5 (Abhydrolase Domain-Containing 5), also known as CGI-58. is a multifunctional protein critical in lipid metabolism. It acts as a coactivator of adipose triglyceride lipase (ATGL), facilitating the hydrolysis of triglycerides stored in lipid droplets. ABHD5 mutations are linked to Chanarin-Dorfman syndrome, a rare autosomal recessive disorder characterized by excessive lipid accumulation in multiple tissues. The ABHD5 antibody is a key tool for studying its expression, localization, and interactions in metabolic pathways. Researchers use it in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate ABHD5's role in lipolysis, autophagy, and intracellular lipid droplet regulation. Its applications extend to exploring metabolic diseases, including obesity, diabetes, and non-alcoholic fatty liver disease (NAFLD). The antibody also aids in elucidating ABHD5's non-canonical functions, such as modulating signaling pathways and membrane trafficking. Validating antibody specificity is essential, as ABHD5 shares structural homology with other hydrolases. Recent studies highlight its potential as a therapeutic target, driving demand for reliable ABHD5 detection reagents in both basic and translational research contexts.

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