| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | T bet; T box 21; T PET; TBET; TBLYM; Tbx21; TPET; Transcription factor TBLYM;;TBX21 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human TBX21 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于TBX21抗体的参考文献(基于公开文献信息概括)：
1. **文献名称**：*T-bet is a STAT1-induced regulator of IL-12R expression in naïve CD4+ T cells*
**作者**：Afkarian M, et al.
**摘要**：该研究利用TBX21抗体(抗T-bet)通过Western blot和染色质免疫沉淀(ChIP)证明，T-bet通过调控IL-12受体表达促进Th1细胞分化，揭示了其在适应性免疫中的关键作用。
2. **文献名称**：*T-bet controls autoimmune inflammation by suppressing Th17 cell differentiation*
**作者**：Lazarevic V, et al.
**摘要**：通过流式细胞术和免疫荧光染色(使用TBX21抗体)，研究发现T-bet通过抑制Th17细胞分化来限制自身免疫性炎症，为多发性硬化等疾病提供了治疗靶点。
3. **文献名称**：*Differential expression of T-bet in tumor-infiltrating lymphocytes predicts cancer prognosis*
**作者**：Kim J, et al.
**摘要**：该文献通过免疫组化(使用TBX21抗体)分析肿瘤微环境中T细胞的T-bet表达水平，发现高表达T-bet的CD8+ T细胞与癌症患者生存率改善显著相关。
4. **文献名称**：*T-bet deficiency attenuates airway hyperresponsiveness in experimental asthma*
**作者**：Finotto S, et al.
**摘要**：研究利用TBX21基因敲除小鼠及抗体检测，证实T-bet通过调控Th1/Th2平衡参与哮喘发病，缺乏T-bet可减轻气道高反应性。
(注：以上内容为基于领域知识的文献概括，具体文献请通过学术数据库检索验证。)
The TBX21 antibody is a crucial tool in immunological research, targeting the T-box transcription factor TBX21 (also known as T-bet). Encoded by the *TBX21* gene, T-bet is a master regulator of T helper 1 (Th1) cell differentiation and function, driving the production of interferon-gamma (IFN-γ) while suppressing Th2 and Th17 pathways. It binds to DNA via a conserved T-box domain, activating Th1-associated genes and repressing competing lineage-specific transcription factors like GATA3. TBX21 is expressed in immune cells, including CD4+ T cells, NK cells, and B cells, and plays roles in autoimmune diseases, infections, and cancer immunology.
Antibodies against TBX21 enable detection and quantification of T-bet protein in techniques such as Western blotting, immunohistochemistry, and flow cytometry. They are vital for studying Th1-mediated immune responses, inflammatory conditions (e.g., multiple sclerosis, rheumatoid arthritis), and therapeutic targeting. Commercial TBX21 antibodies are typically validated for specificity, often through knockout controls or siRNA-based silencing. Challenges include cross-reactivity with homologous proteins or epitope masking in fixed tissues. Recent studies also explore T-bet's role in B cell class-switching and exhausted T cells in chronic infections, underscoring the antibody's continued relevance in advancing immune mechanism research.
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