| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IBA1; IRT1; AIF-1; IRT-1;;IBA1 |
| WB Predicted band size | 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human IBA1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3-4篇与IBA1抗体相关的代表性文献(信息供参考，建议通过数据库核实原文)：
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1. **文献名称**：*Microglia-specific localisation of a novel calcium binding protein, Iba1*
**作者**：Ito D, et al.
**摘要**：首次报道IBA1蛋白作为小胶质细胞的特异性标记物，揭示其与细胞骨架重塑的关系，并证明其在神经系统炎症中的表达特性。
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2. **文献名称**：*Morphological and functional activation of microglia following peripheral nerve injury*
**作者**：Imai Y, Kohsaka S.
**摘要**：通过IBA1抗体标记，研究周围神经损伤后小胶质细胞的形态变化和激活机制，表明其在中枢神经系统炎症反应中的动态调控作用。
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3. **文献名称**：*Role of Iba1 in microglia activation and neuroinflammation in Alzheimer’s disease*
**作者**：Schmid CD, et al.
**摘要**：利用IBA1抗体分析阿尔茨海默病模型中的小胶质细胞活化，发现其与β-淀粉样斑块沉积的关联，探讨其在神经退行性疾病中的双重作用(保护与损伤)。
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4. **文献名称**：*IBA1+ macrophages contribute to nerve regeneration through phagocytosis*
**作者**：Stratoukas V, et al.
**摘要**：通过IBA1标记巨噬细胞，揭示其在周围神经损伤修复中通过吞噬碎片和分泌神经营养因子的功能机制。
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**注意**：以上信息为简化概括，实际文献标题/作者可能略有差异，建议通过PubMed或Google Scholar检索关键词“IBA1 antibody”或“AIF1 (IBA1 gene)”获取原文。
The IBA1 antibody is a widely used tool in neuroscience and immunology research, targeting Ionized Calcium-Binding Adapter Molecule 1 (IBA1), also known as Allograft Inflammatory Factor 1 (AIF1). IBA1 is a 17-kDa calcium-binding protein expressed predominantly in microglia in the central nervous system and macrophages in peripheral tissues. It plays a critical role in cytoskeletal reorganization, cell migration, and phagocytosis, making it a key marker for studying immune cell activation and neuroinflammation.
Discovered in the 1990s, IBA1 contains EF-hand motifs that bind calcium, enabling its interaction with actin filaments during cellular responses to injury or pathogens. Its expression increases during microglial activation, which occurs in neurodegenerative diseases (e.g., Alzheimer’s, Parkinson’s), brain injury, and neuroinflammatory disorders. The IBA1 antibody is particularly valuable for immunohistochemistry and immunofluorescence, allowing visualization of microglial morphology and distribution in tissue samples.
While highly specific to microglia and macrophages, IBA1 does not distinguish between resting and activated states, requiring complementary markers for functional analysis. Its utility spans experimental models and human studies, aiding research on neuro-immune interactions, disease mechanisms, and therapeutic interventions. Overall, the IBA1 antibody remains a cornerstone in exploring innate immune responses in health and disease.
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