| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | bHLHc3; cb553; MYF4; MYOG; Myogenic factor 4;;Myogenin |
| WB Predicted band size | Calculated MW: 25 kDa ; Observed MW: 36 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Myogenin |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Myogenin抗体的参考文献示例(注：以下为模拟示例，实际文献需通过学术数据库获取)：
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1. **文献名称**: *Myogenin: A reliable marker in the diagnosis of rhabdomyosarcoma in children and adolescents*
**作者**: Dias P, Parham DM, Shapiro DN
**摘要**: 研究验证了Myogenin抗体在儿童横纹肌肉瘤诊断中的特异性，表明其高表达可作为区别于其他小圆细胞肿瘤的关键标志物，支持其在病理学中的诊断价值。
2. **文献名称**: *Antibody-based profiling of myogenin expression during skeletal muscle development*
**作者**: Wright WE, Sassoon DA, Lin VK
**摘要**: 通过Myogenin抗体分析小鼠骨骼肌发育过程，揭示Myogenin在肌细胞分化和肌纤维形成中的动态表达模式，证实抗体在发育生物学研究中的可靠性。
3. **文献名称**: *Comparative analysis of MyoD1 and myogenin antibodies in skeletal muscle differentiation*
**作者**: Rudnicki MA, Le Grand F, Kuang S
**摘要**: 比较Myogenin与MyoD1抗体在肌肉分化标记中的应用，发现Myogenin在晚期分化阶段特异性更强，适用于体外成肌实验及疾病模型研究。
4. **文献名称**: *Validation of myogenin antibody for murine muscle regeneration studies*
**作者**: Yun K, Wold B
**摘要**: 验证Myogenin抗体在小鼠肌肉再生模型中的适用性，确认其在Western blot和免疫组化中的高灵敏度，支持其在肌肉损伤修复机制研究中的应用。
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建议通过PubMed、Google Scholar等平台，以“Myogenin antibody”、“diagnostic use”或“muscle differentiation”为关键词检索更多实际文献。
Myogenin, a member of the myogenic regulatory factor (MRF) family, is a key transcription factor regulating skeletal muscle development and differentiation. Encoded by the *MYOG* gene, it belongs to the basic helix-loop-helix (bHLH) superfamily and partners with E-proteins to activate muscle-specific gene expression. Myogenin is essential for myoblast fusion into multinucleated myotubes during embryogenesis and muscle regeneration, with its expression tightly linked to terminal differentiation stages.
Myogenin antibodies are widely used in research and diagnostics to detect myogenin protein levels, primarily via immunohistochemistry (IHC), Western blotting, or immunofluorescence. These antibodies help identify myogenic cells in developmental studies, muscle injury models, and stem cell differentiation assays. Clinically, myogenin IHC is a diagnostic marker for alveolar rhabdomyosarcoma, a pediatric soft tissue cancer, where nuclear staining distinguishes it from other small round blue cell tumors.
Recent studies also explore myogenin's role in muscle atrophy, metabolic disorders, and regenerative therapies. Antibody specificity is critical, as cross-reactivity with other MRFs (e.g., MyoD, Myf5) can occur. Validated myogenin antibodies enable precise tracking of muscle lineage commitment, offering insights into muscular dystrophies, sarcopenia, and therapeutic strategies for muscle repair.
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