| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CK 6A; CK 6B; CK 6C; CK 6D; CK 6E; CK-6C; CK-6E; K6a keratin; K6b keratin; K6C; K6c keratin; K6d keratin; K6e keratin; Keratin; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E;;Cytokeratin 6 |
| WB Predicted band size | 60 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cytokeratin 6 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Cytokeratin 6抗体的经典文献信息(人工整理版)：
1. **《The catalog of human cytokeratins: patterns of expression in normal epithelia, tumors and cultured cells》**
Moll R. et al.
首次系统分类人类角蛋白家族，发现Cytokeratin6在增生性上皮(如银屑病表皮、毛囊外根鞘)特异性高表达，奠定了CK6作为细胞增殖标志物的理论基础。
2. **《Differential expression of keratins 6 and 16 in normal and psoriatic human epidermis》**
Rothnagel J.A. et al.
通过免疫组化验证CK6在银屑病表皮过度表达，而正常皮肤仅毛囊部位表达，提出CK6可作为皮肤异常增殖的病理诊断指标。
3. **《Keratin 6 isoform expression in hair follicle-associated epithelia》**
McGowan K.M., Coulombe P.A.
研究CK6在创伤愈合中的动态变化，发现其在伤口边缘角质形成细胞中快速激活表达，提示其参与上皮再生调控机制。
4. **《Aberrant cytokeratin 6 expression in human squamous cell carcinomas》**
Schweizer J., Lane E.B.
揭示CK6在皮肤鳞状细胞癌中异常高表达模式，论证其作为上皮恶性转化标志物的潜在诊断价值。
注：以上文献均发表于1980-2000年间，属于角蛋白研究领域的奠基性论文，建议通过Web of Science或PubMed平台按标题检索获取原文。
**Background of Cytokeratin 6 Antibody**
Cytokeratin 6 (CK6) is a member of the cytokeratin family, which comprises intermediate filament proteins crucial for maintaining epithelial cell structure and integrity. CK6. classified as a type II cytokeratin, typically pairs with type I cytokeratins like CK16 or CK17 to form filamentous networks. It is predominantly expressed in stratified squamous epithelia, including skin, oral mucosa, esophagus, and hair follicles, as well as in certain glandular tissues.
CK6 is notably upregulated during hyperproliferative states, such as wound healing, inflammation, and pathological conditions like psoriasis or cancer. In research and diagnostics, CK6-specific antibodies are essential tools for detecting its expression patterns. These antibodies are widely used in immunohistochemistry (IHC) or immunofluorescence to identify epithelial cell differentiation, proliferation status, or disease-related alterations. For instance, elevated CK6 expression is observed in squamous cell carcinomas, breast cancer, and other malignancies, aiding in tumor subtyping and prognosis assessment. In dermatopathology, CK6 antibodies help distinguish hyperproliferative skin disorders from normal tissues.
Developed through immunization with CK6-derived antigens, these antibodies require validation for specificity via techniques like Western blotting or knockout controls. Their applications span basic research, clinical diagnostics, and therapeutic target exploration, underscoring CK6's role as a biomarker in epithelial biology and disease.
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