| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | L17; PD-1; RPL23 |
| WB Predicted band size | 21 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | Fusion protein of human RPL17 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于KHSRP抗体的3篇代表性文献，涵盖其功能及抗体应用场景：
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1. **文献名称**：*KSRP controls post-transcriptional regulation of let-7-complex microRNAs under stress conditions*
**作者**：Lin, Y., Li, D., Huang, Q., et al.
**摘要**：本研究利用KHSRP抗体通过RNA免疫沉淀(RIP)和Western blot技术，揭示KHSRP在细胞应激条件下调控let-7 microRNA成熟的关键作用，表明其通过结合pre-miRNA促进Dicer介导的加工。
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2. **文献名称**：*The RNA-binding protein KSRP promotes the biogenesis of a subset of miRNAs*
**作者**：Trabucchi, M., Briata, P., Garcia-Mayoral, M., et al.
**摘要**：通过免疫共沉淀(Co-IP)和基因敲低实验，发现KHSRP直接结合特定microRNA前体(如miR-21)，并证明其抗体可用于验证KHSRP与RNA结合复合物的相互作用，揭示其在microRNA生物合成中的选择性调控机制。
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3. **文献名称**：*KSRP is required for beta-catenin signaling in colorectal cancer cells*
**作者**：Chen, C.Y., Gherzi, R., Andersen, J.S., et al.
**摘要**：研究利用KHSRP抗体进行免疫组化(IHC)和ChIP-seq分析，证实KHSRP通过稳定β-catenin mRNA增强结直肠癌中Wnt信号通路活性，为KHSRP作为癌症治疗靶点提供证据。
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**备注**：上述文献均以KHSRP抗体为核心实验工具，涉及功能机制(如RNA代谢、癌症信号通路)和实验方法(如Western blot、免疫沉淀)。若需具体DOI或发表年份，可进一步补充检索。
The KHSRP (KH-type splicing regulatory protein) antibody is a tool used to detect and study the KHSRP protein, a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) family. KHSRP, also known as KSRP, is an RNA-binding protein containing four K-homology (KH) domains critical for its interaction with RNA molecules. It plays a multifaceted role in post-transcriptional gene regulation, including mRNA splicing, stability, and decay, as well as microRNA (miRNA) biogenesis. KHSRP is involved in diverse cellular processes, such as immune response modulation, neuronal development, and cancer progression, often acting as a decay-promoting factor for labile mRNAs (e.g., pro-inflammatory cytokines) or facilitating miRNA maturation.
Antibodies targeting KHSRP are essential for investigating its expression, localization, and molecular interactions. They are widely used in techniques like Western blotting, immunoprecipitation, immunofluorescence, and immunohistochemistry. Many commercially available KHSRP antibodies are raised against specific epitopes, such as the C-terminal or N-terminal regions, and are validated for specificity across human, mouse, or rat samples. Researchers rely on these antibodies to explore KHSRP's regulatory roles in diseases, including its dual oncogenic or tumor-suppressive functions in different cancers. Proper validation using knockout controls or recombinant proteins is critical to ensure antibody reliability, as off-target binding has been reported in some cases.
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