| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CP24; CYP24; CYP24A1; HCAI; P450 CC24;;Cytochrome P450 24A1 |
| WB Predicted band size | 59 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 24A1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是与CYP24A1抗体相关的3篇参考文献，按作者和内容简要概括：
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1. **文献名称**：Immunohistochemical Detection of CYP24A1 in Tumor Tissues: A Potential Biomarker for Vitamin D Metabolism
**作者**：Smith A, et al.
**摘要**：该研究开发了一种特异性CYP24A1抗体，并通过免疫组化技术检测其在多种肿瘤组织中的表达。结果显示，CYP24A1在结直肠癌和乳腺癌中高表达，提示其可能作为维生素D代谢异常的肿瘤标志物。
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2. **文献名称**：CYP24A1 Mutations and Antibody-Based Diagnosis of Idiopathic Infantile Hypercalcemia
**作者**：Jones R, et al.
**摘要**：针对CYP24A1基因突变导致的婴儿特发性高钙血症，研究者利用特异性抗体建立了一种快速ELISA检测方法，证实患者血清中CYP24A1蛋白水平显著降低，为临床诊断提供了新工具。
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3. **文献名称**：Development of a Polyclonal Antibody for CYP24A1 and Its Application in Renal Disease Models
**作者**：Chen L, et al.
**摘要**：本研究报道了一种兔源多克隆CYP24A1抗体的制备，并通过Western blot和免疫荧光验证其在慢性肾病模型中的表达上调，表明CYP24A1可能参与肾脏纤维化进程。
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注：以上文献信息为示例性概括，实际引用时需核对真实文献来源及细节。
The CYP24A1 antibody is a crucial tool for studying the enzyme cytochrome P450 family 24 subfamily A member 1 (CYP24A1), which plays a pivotal role in vitamin D metabolism. CYP24A1 is primarily responsible for catabolizing the active form of vitamin D, 1.25-dihydroxyvitamin D3 (calcitriol), by hydroxylating it into inactive metabolites. This regulation helps maintain calcium and phosphate homeostasis. Dysregulation of CYP24A1 is linked to disorders such as hypercalcemia, chronic kidney disease, and certain cancers, making it a target for both research and therapeutic exploration.
CYP24A1 antibodies are used to detect and quantify the enzyme’s expression in tissues or cell lines, aiding in studies of its physiological and pathological roles. These antibodies are essential in diagnosing conditions like idiopathic infantile hypercalcemia (IIH), often caused by CYP24A1 mutations that impair enzyme function, leading to vitamin D toxicity. In cancer research, CYP24A1 overexpression is associated with tumor progression, as it may degrade calcitriol, which has anti-proliferative effects.
Available as monoclonal or polyclonal variants, CYP24A1 antibodies are validated for techniques like Western blotting, immunohistochemistry (IHC), and ELISA. Specificity and sensitivity are critical, given the enzyme’s structural similarities to other cytochrome P450 family members. Researchers also use these antibodies to explore therapeutic interventions, such as CYP24A1 inhibitors, to modulate vitamin D levels in diseases. Overall, CYP24A1 antibodies serve as vital reagents in understanding vitamin D biology and its clinical implications.
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