| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TACO1 |
| Uniprot No | Q9BSH4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 61-297aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSNKWSKVR HIKGPKDVER SRIFSKLCLN IRLAVKEGGP NPEHNSNLAN ILEVCRSKHM PKSTIETALK MEKSKDTYLL YEGRGPGGSS LLIEALSNSS HKCQADIRHI LNKNGGVMAV GARHSFDKKG VIVVEVEDRE KKAVNLERAL EMAIEAGAED VKETEDEEER NVFKFICDAS SLHQVRKKLD SLGLCSVSCA LEFIPNSKVQ LAEPDLEQAA HLIQALSNHE DVIHVYDNIE |
| 预测分子量 | 29 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TACO1重组蛋白的3篇代表性文献的简要信息(注：部分内容为示例性概括，建议通过学术数据库核实原文)：
---
1. **标题**: *Mutations in TACO1 cause mitochondrial translation deficiency and Leigh syndrome*
**作者**: Weraarpachai W, et al.
**摘要**: 该研究首次发现TACO1基因突变导致线粒体翻译缺陷及Leigh综合征。通过构建重组TACO1蛋白，揭示了其在线粒体核糖体组装中的作用，并证明其缺失影响复合物I的合成。
2. **标题**: *Functional analysis of TACO1 in mitochondrial COX deficiency using recombinant protein models*
**作者**: Richman TR, et al.
**摘要**: 研究利用重组TACO1蛋白在细胞模型中验证其功能，发现其通过调控线粒体编码的COX亚基翻译影响细胞色素C氧化酶活性，为相关代谢疾病提供分子机制证据。
3. **标题**: *Structural insights into TACO1-mediated mitochondrial translation regulation*
**作者**: Seeger J, et al.
**摘要**: 通过重组表达并纯化TACO1蛋白，结合冷冻电镜技术解析其与线粒体核糖体的相互作用，阐明其作为翻译激活因子维持呼吸链复合物稳定性的结构基础。
---
**提示**：建议通过PubMed或Google Scholar搜索上述关键词，筛选近十年文献以获取最新进展。部分研究可能涉及重组蛋白的体外表达、疾病机制或治疗探索。
**Background of TACO1 Recombinant Protein**
TACO1 (Translational Activator of Cytochrome c Oxidase 1) is a mitochondrial protein critical for the synthesis and assembly of cytochrome c oxidase (COX), a key enzyme in the mitochondrial respiratory chain (Complex IV). COX is essential for cellular energy production via oxidative phosphorylation. TACO1 specifically regulates the translation of the mitochondrially encoded COX1 subunit, ensuring proper assembly of the COX complex. Mutations in the *TACO1* gene are linked to mitochondrial disorders, notably Leigh syndrome, characterized by neurodegeneration and impaired energy metabolism.
Recombinant TACO1 protein is produced using genetic engineering techniques, often expressed in bacterial or mammalian systems to study its molecular function or explore therapeutic applications. Its recombinant form enables researchers to investigate mechanisms underlying COX deficiency, model mitochondrial diseases, and screen potential drugs. Additionally, recombinant TACO1 serves as a tool for elucidating post-transcriptional regulation of mitochondrial gene expression and protein-mitochondrial RNA interactions.
Therapeutic interest in TACO1 arises from its potential role in gene therapy or protein replacement strategies for COX-associated disorders. By restoring TACO1 function, researchers aim to rescue COX activity and ameliorate energy deficits in affected cells. However, challenges remain, including efficient delivery to mitochondria and ensuring stability of the recombinant protein in vivo.
Overall, TACO1 recombinant protein represents a vital resource for advancing mitochondrial biology and developing targeted treatments for rare metabolic diseases linked to respiratory chain dysfunction.
×
