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Rabbit Monoclonal PCK1 Antibody

  • 中文名： PCK1抗体
  • 别    名： GTP; PCK1; PEP carboxykinase; PEPCK1; PEPCKC;;PCK1
货号: IPDX18476
Price： ￥1280
数量：
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验证与应用

应用及物种
WB 1/1000-1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 咨询技术 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesGTP; PCK1; PEP carboxykinase; PEPCK1; PEPCKC;;PCK1
WB Predicted band size69 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman
ImmunogenA synthesized peptide derived from human PCK1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是3条关于PCK1抗体的示例参考文献(内容为模拟示例，建议通过学术数据库验证准确性)：

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1. **文献名称**：*"PCK1-mediated metabolic reprogramming in hepatocellular carcinoma"*

**作者**：Li, X. et al.

**摘要**：研究利用PCK1特异性抗体(货号AB123)通过Western blot和免疫组化分析肝癌组织中PCK1的表达水平，发现PCK1下调与肝癌细胞糖异生能力减弱相关，提示其在肝癌代谢重编程中的作用。

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2. **文献名称**：*"Role of PCK1 in glucose homeostasis and type 2 diabetes"*

**作者**：Zhang, Y. & Wang, H.

**摘要**：通过PCK1抗体(品牌CellSignaling，克隆号C45D8)进行肝组织免疫荧光染色，证实PCK1在糖尿病模型中表达异常，其活性受胰岛素信号通路调控，影响肝脏葡萄糖输出。

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3. **文献名称**：*"PCK1 deficiency exacerbates lipid accumulation in non-alcoholic fatty liver disease"*

**作者**：Kim, S. et al.

**摘要**：研究使用PCK1抗体(品牌Santa Cruz，sc-55561)进行Western blot和免疫沉淀实验，发现PCK1缺失导致小鼠肝脏脂质代谢紊乱，并揭示了其与AMPK通路的相互作用机制。

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**注意**：以上文献信息为示例，实际引用需查询真实数据库(如PubMed、Web of Science)。建议使用关键词“PCK1 antibody”或“phosphoenolpyruvate carboxykinase 1”结合实验技术(Western blot/IHC)进行检索。

背景信息

The PCK1 antibody is a crucial tool for studying phosphoenolpyruvate carboxykinase 1 (PCK1), a rate-limiting enzyme in gluconeogenesis that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate. Primarily expressed in the liver and kidneys, PCK1 plays a central role in glucose homeostasis by regulating hepatic glucose output. Dysregulation of PCK1 is linked to metabolic disorders such as type 2 diabetes, obesity, and non-alcoholic fatty liver disease (NAFLD). Researchers use PCK1 antibodies in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to detect protein expression levels, cellular localization, and tissue distribution. These antibodies help investigate PCK1's role in metabolic pathways, hormonal regulation (e.g., insulin, glucagon), and its interaction with signaling molecules like AMPK and mTOR. Additionally, PCK1 antibodies are employed in studying cancer metabolism, as some tumors exploit gluconeogenic pathways for growth. Commercial PCK1 antibodies are typically raised against specific epitopes, often validated for cross-reactivity across human, mouse, and rat models. Recent studies also explore PCK1's non-metabolic functions, including apoptosis regulation and redox balance, expanding its therapeutic relevance. Proper validation via knockout controls or siRNA is essential to ensure antibody specificity in experimental settings.

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