| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Amp2; MAP2; Metap2; MNPEP; p67; p67eIF2;;METAP2 |
| WB Predicted band size | Calculated MW: 53 kDa ; Observed MW: 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human METAP2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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1. **"Inhibition of angiogenesis by the antifungal drug itraconazole"** by N. S. Chong et al. (2007)
- 摘要：该研究探讨了抗真菌药物伊曲康唑通过靶向MetAP2抑制血管生成的机制，利用MetAP2抗体证实了药物处理后人脐静脉内皮细胞中MetAP2蛋白水平的下降，揭示了其抗肿瘤潜力。
2. **"Methionine aminopeptidase-2 regulates inflammatory response in macrophages via NF-κB signaling"** by J. Li et al. (2015)
- 摘要：通过MetAP2特异性抗体检测，研究发现巨噬细胞中MetAP2通过调控NF-κB通路参与炎症反应，基因敲除或抗体介导的蛋白抑制显著降低促炎因子表达。
3. **"Targeting methionine aminopeptidase 2 for cancer therapy: discovery of TNP-470 and beyond"** by K. E. Yeh et al. (2006)
- 摘要：综述了MetAP2作为抗肿瘤靶点的研究进展，提及利用抗体分析TNP-470等抑制剂对MetAP2的抑制作用及其在抑制内皮细胞增殖中的效果。
4. **"MetAP2 modulates mitochondrial respiration and hepatic lipid metabolism in non-alcoholic fatty liver disease"** by M. Zhang et al. (2020)
- 摘要：通过Western blot(MetAP2抗体)和基因沉默实验，证明MetAP2通过调节线粒体功能影响肝脏脂质代谢，其表达水平与脂肪肝严重程度正相关。
Methionine aminopeptidase 2 (MetAP2) is a conserved enzyme that plays a critical role in post-translational protein modification by catalyzing the removal of initiator methionine residues from nascent polypeptides. This process is essential for protein maturation, stability, and function. MetAP2 is also implicated in regulating cellular proliferation, angiogenesis, and tumor growth, making it a potential therapeutic target in cancer and inflammatory diseases.
Antibodies targeting MetAP2 are valuable tools for studying its expression, localization, and biological functions. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to quantify MetAP2 levels in tissues or cultured cells. Research has shown that MetAP2 overexpression correlates with certain cancers, while its inhibition (e.g., via fumagillin analogs) suppresses endothelial cell growth, highlighting its role in pathological angiogenesis.
MetAP2 antibodies also aid in validating the efficacy of gene-silencing approaches (e.g., siRNA) or pharmacological inhibitors in preclinical models. Additionally, studies link MetAP2 dysfunction to metabolic disorders, broadening its relevance beyond oncology. By enabling precise detection and functional analysis, these antibodies contribute to understanding MetAP2's dual role in normal physiology and disease, supporting drug development and biomarker discovery.
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