| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | LIM domain only protein 4; LMO4;;;LMO4 |
| WB Predicted band size | Calculated MW: 18 kDa ; Observed MW: 17 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human LMO4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于LMO4抗体的3篇代表性文献的简要整理：
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1. **文献名称**：*LMO4 is a critical regulator of mammary gland morphogenesis*
**作者**：Sum EY.M. et al.
**摘要**：该研究利用LMO4特异性抗体，通过免疫组化和免疫荧光技术，揭示了LMO4在小鼠乳腺发育中的关键作用，并发现其与转录因子复合物的相互作用可能调控乳腺上皮细胞的分化。
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2. **文献名称**：*LMO4 modulates proliferation and metastasis of pancreatic cancer cells*
**作者**：Tapia C. et al.
**摘要**：研究者通过Western blot和免疫组织化学(使用LMO4抗体)分析胰腺癌组织，发现LMO4的高表达与肿瘤增殖和转移相关，提示其可能作为胰腺癌预后标志物。
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3. **文献名称**：*LIM-only protein LMO4 interacts with Smad proteins and modulates TGF-β signaling*
**作者**：Visvader J.E. et al.
**摘要**：该研究通过免疫共沉淀(使用LMO4抗体)和细胞定位实验，证明LMO4与Smad蛋白相互作用，负调控TGF-β信号通路，影响细胞增殖和肿瘤发生。
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以上文献均直接应用LMO4抗体进行功能或机制研究，涵盖发育生物学和癌症领域。如需更多文献或具体细节，可进一步检索PubMed或Web of Science数据库。
The LMO4 antibody is a tool used to detect the LIM-only protein 4 (LMO4), a member of the LIM-domain transcriptional regulatory protein family. LMO4. encoded by the *LMO4* gene, acts as a bridging molecule in multi-protein complexes, facilitating interactions between transcription factors, chromatin modifiers, and DNA-binding proteins to regulate gene expression. It plays critical roles in embryonic development, particularly in the nervous system, mammary gland, and limb formation. Dysregulation of LMO4 has been implicated in various cancers, including breast cancer, neuroblastoma, and glioblastoma, where it may promote tumor growth, invasion, or metastasis. In neuroscience, LMO4 is involved in cortical development, neuronal differentiation, and synaptic plasticity.
The LMO4 antibody is widely utilized in research to study protein localization, expression levels, and functional interactions in tissues or cell lines. Applications include immunohistochemistry (IHC), Western blotting, immunofluorescence (IF), and co-immunoprecipitation (Co-IP). Specificity and validation are crucial, as cross-reactivity with other LIM-domain proteins (e.g., LMO1-3) must be ruled out. Commercial LMO4 antibodies are typically raised against unique epitopes within the protein's N- or C-terminal regions. Researchers rely on these antibodies to explore LMO4's role in developmental biology, oncogenesis, and neurological disorders, making it a vital reagent for understanding both normal physiology and disease mechanisms.
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