| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | VAP33; Vapa;;VAMP A |
| WB Predicted band size | 28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human VAMP A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于VAPA抗体的研究文献概览(基于近年研究，信息经合理整合)：
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1. **文献名称**: *VAPA regulates ER-mitochondrial contact sites and metabolic homeostasis*
**作者**: Lee S, et al. (2022)
**摘要**: 本研究利用VAPA特异性抗体，通过免疫共沉淀和免疫荧光技术，揭示了VAPA蛋白在内质网-线粒体膜接触位点的定位，并证明其缺失会导致脂代谢紊乱和胰岛素抵抗。抗体检测显示VAPA与胆固醇转运蛋白STARD3存在直接互作。
2. **文献名称**: *VAPA-antibody as a novel biomarker in neurodegenerative disorders*
**作者**: Rossi G, et al. (2021)
**摘要**: 研究者开发了高特异性VAPA单克隆抗体，发现帕金森病患者脑脊液中VAPA蛋白水平显著降低。通过Western blot和ELISA验证，提出VAPA抗体可作为神经退行性疾病的潜在诊断标志物。
3. **文献名称**: *HCV NS5A hijacks VAPA through dual interaction mechanisms*
**作者**: Zhang Y, et al. (2020)
**摘要**: 使用VAPA抗体进行免疫共沉淀实验，证实丙肝病毒NS5A蛋白通过FFAT和磷酸化结构域双重结合VAPA，破坏细胞内脂滴运输。研究揭示了病毒劫持宿主VAPA蛋白促进自身复制的分子机制。
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**备注**：以上文献信息为模拟示例，实际文献请通过PubMed/Google Scholar检索关键词如"VAPA antibody application"或结合具体研究领域进行查询。
VAPA (Vesicle-associated Membrane Protein-associated Protein A) is a conserved transmembrane protein primarily localized to the endoplasmic reticulum (ER) and other membranous organelles. It belongs to the VAP protein family, which includes VAPB, and plays a critical role in membrane contact site formation, lipid transfer, and organelle dynamics. VAPA contains a major sperm protein (MSP) domain that interacts with FFAT motifs in lipid-transfer proteins like OSBP and CERT, facilitating non-vesicular transport of lipids such as cholesterol and phosphatidylinositol. This function links VAPA to cellular processes including vesicle trafficking, autophagy, and ER homeostasis.
Antibodies targeting VAPA are essential tools for studying its expression, localization, and interactions in normal physiology and disease contexts. Dysregulation of VAPA has been implicated in neurodegenerative disorders (e.g., amyotrophic lateral sclerosis), cancer progression, and metabolic syndromes. Researchers employ VAPA-specific antibodies in techniques like Western blotting, immunofluorescence, and co-immunoprecipitation to investigate its molecular mechanisms. These antibodies are typically validated using knockout controls to ensure specificity. Recent studies also explore VAPA's role in viral infections, as some pathogens exploit VAPA-mediated lipid transport for replication. The development of high-affinity anti-VAPA antibodies continues to advance research into organelle communication and therapeutic targeting of lipid metabolism pathways.
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