| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | cMaf; maf; MAF2; Proto oncogene c Maf;;c Maf |
| WB Predicted band size | Calculated MW: 38 kDa ; Observed MW: 44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human c Maf |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于c-Maf抗体的3篇参考文献示例(文献信息为虚构示例，实际文献需根据具体数据库检索)：
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1. **文献名称**: *"c-Maf promotes multiple myeloma cell growth through transcriptional regulation of cyclin D2"*
**作者**: Hurt EM et al.
**摘要**: 研究利用c-Maf特异性抗体(如ChIP-seq和Western blot)揭示c-Maf在多发性骨髓瘤中过表达，并通过调控cyclin D2促进肿瘤增殖，为靶向治疗提供依据。
2. **文献名称**: *"c-Maf is essential for IL-10 gene expression in Th2 cells"*
**作者**: Kim JI et al.
**摘要**: 通过c-Maf抗体阻断实验和基因敲除模型，证明c-Maf在Th2细胞中直接激活IL-10转录，调控免疫应答，抗体用于染色质免疫共沉淀(ChIP)验证结合位点。
3. **文献名称**: *"Immunohistochemical detection of c-Maf in plasma cell disorders"*
**作者**: Sasaki A et al.
**摘要**: 使用抗c-Maf单克隆抗体对患者组织样本进行免疫组化分析，发现c-Maf高表达与多发性骨髓瘤不良预后相关，支持其作为临床病理标志物。
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**注**：以上为示例，实际引用时请通过PubMed/Google Scholar等平台检索真实文献(关键词：c-Maf antibody, immunohistochemistry, ChIP-seq)。
The c-Maf antibody is a crucial tool in studying the c-Maf transcription factor, a member of the large Maf protein family. c-Maf plays pivotal roles in cellular differentiation, immune regulation, and oncogenesis. It binds to Maf recognition elements (MAREs) in DNA, regulating gene expression involved in Th17 cell differentiation, interleukin production (e.g., IL-4. IL-10), and plasma cell development. Dysregulation of c-Maf is linked to multiple myeloma, rheumatoid arthritis, and other pathologies.
c-Maf antibodies are widely used to detect and quantify protein expression in research settings. These antibodies are typically generated against specific epitopes, such as the N-terminal or C-terminal regions, and validated for applications like Western blotting (WB), immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry. Monoclonal antibodies offer high specificity, while polyclonal versions may detect broader isoforms but require rigorous validation to minimize cross-reactivity with related Maf proteins (e.g., MafB, MafA).
In cancer research, c-Maf antibodies help identify its overexpression in tumors, aiding prognosis and therapeutic targeting. In immunology, they elucidate mechanisms of T-cell polarization and cytokine regulation. Commercial antibodies often include validation data from knockout controls or siRNA-treated samples to confirm specificity. Researchers must consider species reactivity (human, mouse, rat) and compatibility with experimental models when selecting c-Maf antibodies.
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