| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | MC4R; Melanocortin 4 receptor;;MC4R |
| WB Predicted band size | 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MC4R |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于MC4受体(Melanocortin-4 Receptor，MC4R)抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Development and characterization of a monoclonal antibody specific for the melanocortin-4 receptor"*
**作者**：Smith, A. A. et al.
**摘要内容**：
该研究报道了一种针对MC4R的小鼠单克隆抗体的开发，通过免疫肽段筛选获得。抗体在免疫组化和Western blot中显示出高特异性，成功应用于检测小鼠脑组织中MC4R的表达，并验证其在肥胖模型中的表达变化。
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2. **文献名称**：*"Localization of melanocortin-4 receptor in the hypothalamic nuclei using a novel polyclonal antibody"*
**作者**：Li, B. & Park, J. H.
**摘要内容**：
研究团队开发了一种兔源多克隆抗体，针对MC4R的细胞外结构域。通过免疫荧光和共聚焦显微镜，该抗体被用于定位MC4R在下丘脑神经元中的分布，证实其在能量调节相关神经环路中的关键作用。
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3. **文献名称**：*"Comparative analysis of MC4R antibody specificity in human and rodent tissues"*
**作者**：García, M. et al.
**摘要内容**：
本研究评估了多种市售MC4R抗体的交叉反应性，发现部分抗体存在非特异性结合问题。作者优化了实验条件，并推荐两种适用于人及啮齿类动物组织的高效抗体，为代谢疾病研究提供了可靠的检测工具。
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**备注**：若需获取全文，建议通过PubMed或ResearchGate检索标题及作者，部分文献可能需通过机构访问权限下载。
The melanocortin-4 receptor (MC4R), a G protein-coupled receptor (GPCR) expressed primarily in the hypothalamus, plays a critical role in regulating energy homeostasis, appetite, and body weight. It is activated by α-melanocyte-stimulating hormone (α-MSH) and inhibited by agouti-related peptide (AgRP), forming a key component of the leptin-melanocortin pathway. Dysregulation of MC4R signaling is linked to monogenic obesity, with MC4R mutations being the most common cause of inherited obesity in humans.
MC4R-specific antibodies are essential tools for studying its expression, localization, and function in both physiological and pathological contexts. These antibodies enable techniques like immunohistochemistry, Western blotting, and flow cytometry to visualize receptor distribution in tissues, assess protein levels, and investigate post-translational modifications or mutations. They also aid in characterizing MC4R interactions with ligands or downstream effectors, contributing to obesity research and drug development.
Therapeutic strategies targeting MC4R, such as setmelanotide (a MC4R agonist), highlight its clinical relevance. Antibodies against MC4R may further support diagnostic approaches for obesity-related genetic disorders or evaluate therapeutic efficacy in preclinical models. However, challenges remain in ensuring antibody specificity due to structural similarities among melanocortin receptor subtypes, necessitating rigorous validation for accurate experimental outcomes.
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