| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/200 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Catechol O methyltransferase; COMT;;COMT |
| WB Predicted band size | Calculated MW: 30 kDa ; Observed MW: 24,28 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human COMT |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇涉及COMT抗体的研究文献概览(注：文献名为虚拟示例，实际研究中请核实)：
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1. **文献名称**: *"COMT Antibody Validation in Postmortem Human Brain Tissue"*
**作者**: Tunbridge, E.M. et al.
**摘要**: 本研究使用特异性COMT抗体在人类尸检脑组织中进行Western blot和免疫组化验证，发现抗体能有效区分COMT的膜结合型(MB-COMT)和可溶型(S-COMT)，并揭示了COMT在前额叶皮层中的层特异性表达模式，支持其在精神分裂症中的功能研究。
2. **文献名称**: *"Regional Distribution of Catechol-O-Methyltransferase Protein in Parkinson's Disease"*
**作者**: Slifstein, M. et al.
**摘要**: 通过免疫荧光和ELISA技术结合COMT特异性抗体，发现帕金森病患者纹状体和前额叶区域的COMT蛋白水平显著升高，提示其与多巴胺代谢代偿机制相关，为COMT抑制剂治疗提供依据。
3. **文献名称**: *"Development of a High-Specificity COMT Monoclonal Antibody for Cancer Research"*
**作者**: Chen, J. & Matsumoto, T.
**摘要**: 报道了一种新型COMT单克隆抗体的开发，该抗体通过表位映射确认对COMT异构体具有高选择性，成功应用于乳腺癌细胞系和组织微阵列分析，显示COMT高表达与雌激素受体信号通路存在关联。
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**注**：实际研究中建议通过PubMed或Google Scholar以“COMT antibody + application/validation”为关键词检索真实文献，重点关注抗体验证方法(如敲除/敲低实验验证特异性)及在神经、肿瘤领域的应用研究。
Catechol-O-methyltransferase (COMT) is a critical enzyme involved in the metabolism of catecholamine neurotransmitters, such as dopamine, epinephrine, and norepinephrine. It catalyzes the transfer of a methyl group from S-adenosylmethionine to catechol substrates, regulating neurotransmitter levels and influencing cognitive, emotional, and motor functions. COMT is highly expressed in the prefrontal cortex, liver, and kidneys, and its activity is linked to genetic polymorphisms, notably the Val158Met variant, which affects enzyme stability and substrate affinity. Dysregulation of COMT has been associated with neurological and psychiatric disorders, including schizophrenia, Parkinson’s disease, anxiety, and depression, making it a key target for research in neuropharmacology and genetics.
COMT antibodies are essential tools for studying the enzyme’s expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to quantify COMT levels in tissues or cell cultures, assess its distribution in the brain, and investigate correlations between genetic variants and protein activity. These antibodies also aid in exploring COMT’s role in dopamine-dependent processes, such as working memory and stress response, and in developing therapeutic strategies targeting catecholamine pathways. Validated COMT antibodies ensure specificity for distinct isoforms (soluble vs. membrane-bound) and species, supporting both basic research and clinical studies aimed at understanding neuropsychiatric diseases and personalized medicine approaches.
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