| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 5 HT 7; 5 HT X; 5-HT-7; 5-HT-X; 5-HT7; 5HT7; htr7;;5 HT7 |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human 5 HT7 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于5HT7受体抗体的3-4篇参考文献的简要概括：
1. **文献名称**：*"Characterization of a specific antibody against the 5-HT7 receptor: Immunohistochemical localization in rat brain"*
**作者**：Thomas et al.
**摘要**：该文献通过Western blot和免疫组化技术验证了5HT7受体抗体的特异性，确认其在啮齿类动物脑组织中的表达模式，重点分析了受体在皮层、海马和下丘脑等区域的分布。
2. **文献名称**：*"The 5-HT7 receptor: distribution and functional relevance in brain"*
**作者**：Leopoldo et al.
**摘要**：研究利用特异性抗体结合基因敲除小鼠模型，系统研究了5HT7受体在中枢神经系统中的分布，并探讨其在调节昼夜节律、情绪及认知功能中的作用。
3. **文献名称**：*"Antibody-based detection of 5-HT7 receptor signaling complexes in neuronal cells"*
**作者**：Bhatnagar et al.
**摘要**：通过共聚焦显微技术和免疫沉淀方法，揭示了5HT7受体与G蛋白及β-arrestin的相互作用，抗体被用于定位受体在突触前膜及胞内信号通路的动态变化。
4. **文献名称**：*"5-HT7 receptor antibodies as tools for studying neuropsychiatric disorders"*
**作者**：Méndez-David et al.
**摘要**：研究测试了多种5HT7抗体在人类和动物模型中的交叉反应性，发现其在抑郁症和焦虑症模型中受体表达水平的变化，提示其作为疾病生物标志物的潜力。
这些文献均聚焦于抗体的特异性验证、受体分布及功能研究，为5HT7受体的基础与转化研究提供了技术支持。
The 5-HT7 receptor, a member of the G protein-coupled receptor (GPCR) family, is one of seven serotonin receptor subtypes (5-HT1–7) that mediate the diverse physiological effects of serotonin. Primarily expressed in the central nervous system (CNS), it plays key roles in regulating circadian rhythms, mood, sleep-wake cycles, and cognitive functions. Unlike other serotonin receptors, the 5-HT7 receptor is also found in peripheral tissues, including the gastrointestinal tract and vascular smooth muscle, highlighting its broader functional significance.
Antibodies targeting the 5-HT7 receptor are critical tools for studying its expression, localization, and function. These antibodies are commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to map receptor distribution in brain regions (e.g., hypothalamus, hippocampus) and peripheral tissues. Research leveraging these antibodies has linked 5-HT7 receptor dysregulation to neuropsychiatric disorders (e.g., depression, schizophrenia), migraines, and irritable bowel syndrome.
A major challenge in developing 5-HT7 receptor antibodies lies in ensuring specificity, as the receptor shares structural homology with other serotonin subtypes. High-quality antibodies are validated using knockout (KO) models or siRNA knockdown to confirm minimal cross-reactivity. Recent advances in epitope design and validation protocols have improved reliability, aiding drug discovery efforts targeting 5-HT7 for therapeutic interventions. Despite progress, the receptor’s complex signaling pathways and splice variants necessitate continued refinement of antibody tools to unravel its multifaceted roles in health and disease.
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