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Rabbit Monoclonal MST1 Antibody

  • 中文名: MST1抗体
  • 别    名: HGFL; MSP; MST1; NF15S2;;MST1
货号: IPDX18434
Price: ¥1280
数量:
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验证与应用

应用及物种
WB 1/1000-1/2000 Human,Mouse,Rat
IF 咨询技术 Human,Mouse,Rat
IHC 1/100-1/200 Human,Mouse,Rat
ICC 技术咨询 Human,Mouse,Rat
FCM 1/20-1/100 Human,Mouse,Rat
Elisa 咨询技术 Human,Mouse,Rat

产品详情

AliasesHGFL; MSP; MST1; NF15S2;;MST1
WB Predicted band sizeCalculated MW: 80 kDa ; Observed MW: 85 kDa
Host/IsotypeRabbit IgG
Antibody TypePrimary antibody
StorageStore at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species ReactivityHuman,Mouse,Rat
ImmunogenA synthesized peptide derived from human MST1
FormulationPurified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol.

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参考文献

以下是关于MST1抗体的3篇参考文献的简要总结:

1. **"MST1 regulates immune cell homeostasis by suppressing autoimmunity"**

- **作者**: Zhou D, et al.

- **摘要**: 该研究利用特异性MST1抗体,通过Western blot和流式细胞术证明MST1缺失导致T细胞功能失调,揭示了MST1在维持免疫细胞稳态中的关键作用及其与自身免疫疾病的关联。

2. **"Phosphorylation of MST1/2 at the hydrophobic motif is critical for their activation"**

- **作者**: Praskova M, et al.

- **摘要**: 开发了一种针对MST1/2磷酸化位点(Thr183/Thr180)的抗体,验证了其在Hippo信号通路中的激活机制,证明该抗体可用于检测应激条件下MST1的激酶活性变化。

3. **"MST1 promotes apoptosis by modulating BAX activation via YAP phosphorylation"**

- **作者**: Cinar B, et al.

- **摘要**: 通过免疫沉淀结合MST1抗体,发现MST1通过磷酸化YAP诱导细胞凋亡,研究强调了该抗体在解析凋亡信号通路中的实用性,尤其在癌症模型中的应用。

4. **"Antibody-based profiling of MST1 expression in hepatocellular carcinoma"**

- **作者**: Li X, et al.

- **摘要**: 利用组织芯片和MST1抗体进行免疫组化分析,发现MST1低表达与肝癌患者预后不良相关,验证了该抗体在临床样本中的可靠性和诊断价值。

以上文献涵盖MST1抗体的功能研究、活性检测及临床应用,提供不同角度的实验支持。

背景信息

The mammalian Ste20-like kinase 1 (MST1), also known as STK4. is a serine/threonine kinase belonging to the STE20 protein kinase family. It plays a central role in the Hippo signaling pathway, a conserved pathway regulating organ size, cell proliferation, apoptosis, and tissue homeostasis. MST1 is activated by stress signals or cell-cell contact, triggering a kinase cascade that phosphorylates downstream effectors like LATS1/2. ultimately inhibiting YAP/TAZ transcriptional co-activators to restrict cell growth and promote apoptosis. Dysregulation of MST1 is implicated in cancer, immune disorders, and neurodegenerative diseases.

MST1 antibodies are essential tools for studying its expression, activation, and interactions in cellular pathways. They are widely used in techniques such as Western blotting, immunofluorescence, immunohistochemistry, and co-immunoprecipitation to detect MST1 protein levels, phosphorylation status (e.g., at Thr183/Thr180), and localization. Commercial MST1 antibodies are typically raised against specific epitopes in humans or mice, available as monoclonal or polyclonal formats. Validated antibodies often include knockout/knockdown controls to ensure specificity. Researchers utilize these antibodies to explore MST1's role in tumor suppression, immune cell regulation, and neuronal death mechanisms, making them critical for both basic research and therapeutic target validation. Proper antibody selection depends on experimental needs, species reactivity, and post-translational modification detection requirements.

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