| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CP12; CYP1A2; CYPIA2; P3 450; P450 4; P450 P3;;Cytochrome P450 1A2 |
| WB Predicted band size | 58 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human Cytochrome P450 1A2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Cytochrome P450 1A2(CYP1A2)抗体的参考文献概览，供您参考：
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1. **文献名称**：*"Development and validation of a monoclonal antibody-based ELISA for quantification of CYP1A2 in human liver microsomes"*
**作者**：Smith J, et al.
**摘要**：该研究开发了一种特异性识别CYP1A2的单克隆抗体，并建立了基于ELISA的定量方法，用于检测人肝微粒体中CYP1A2的表达水平，验证了其在药物代谢研究中的应用潜力。
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2. **文献名称**：*"Immunohistochemical localization of CYP1A2 in human tissues using a novel polyclonal antibody"*
**作者**：Chen L, et al.
**摘要**：研究者制备了一种新型多克隆抗体，通过免疫组化技术揭示了CYP1A2在肝脏、胃肠道及脑组织中的分布差异，为研究其组织特异性代谢功能提供工具。
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3. **文献名称**：*"Role of CYP1A2 in coffee-induced metabolism: Insights from antibody-mediated inhibition studies"*
**作者**：Rodriguez-Morato J, et al.
**摘要**：利用CYP1A2抗体抑制酶活性，探讨了咖啡因代谢中CYP1A2的核心作用，证实抗体阻断实验可作为评估药物-食物相互作用的重要方法。
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**注**：以上文献信息为示例性内容，实际引用时需核对真实文献来源(可通过PubMed、Google Scholar等平台搜索关键词：*CYP1A2 antibody* + *应用场景*)。若需具体文献，可补充说明研究方向(如疾病机制、药物代谢等)，我将进一步筛选。
Cytochrome P450 1A2 (CYP1A2) is a member of the cytochrome P450 enzyme family, primarily expressed in the liver and involved in the metabolism of xenobiotics, drugs, and endogenous compounds. It plays a critical role in detoxifying procarcinogens, such as polycyclic aromatic hydrocarbons (PAHs) and heterocyclic amines, while also contributing to the bioactivation of certain toxins. CYP1A2 activity is highly variable among individuals due to genetic polymorphisms, environmental factors (e.g., smoking, diet), and drug interactions, influencing drug efficacy and toxicity risks.
CYP1A2 antibodies are essential tools for studying the enzyme's expression, regulation, and function in both research and clinical settings. These antibodies enable the detection and quantification of CYP1A2 protein levels in tissues or cell lysates using techniques like Western blotting, immunohistochemistry, and ELISA. Researchers use them to investigate CYP1A2 induction mechanisms, particularly its upregulation via the aryl hydrocarbon receptor (AhR) pathway upon exposure to ligands like dioxins or PAHs. Additionally, CYP1A2 antibodies aid in exploring interactions with drugs (e.g., caffeine, clozapine) and assessing disease associations, such as liver disorders, cancer susceptibility, or metabolic syndromes. Their specificity and reliability are crucial for elucidating CYP1A2's role in pharmacology, toxicology, and personalized medicine.
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