| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Chromokinesin; KIF4; KIF4 G1; KIF4A; KIF4G1;;Chromokinesin A |
| WB Predicted band size | Calculated MW: 140 kDa ; Observed MW: 150 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human Chromokinesin A |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KIF4A抗体的3篇参考文献示例(内容为模拟生成，仅供参考)：
1. **文献名称**: "KIF4A as a prognostic biomarker in breast cancer: Expression analysis and functional validation"
**作者**: Zhang et al. (2020)
**摘要**: 本研究通过免疫组化(使用KIF4A特异性抗体)发现KIF4A在乳腺癌组织中高表达，且与患者不良预后相关。体外实验表明，KIF4A敲除可抑制肿瘤细胞增殖和迁移，提示其作为潜在治疗靶点。
2. **文献名称**: "KIF4A regulates DNA repair and chemoresistance in colorectal cancer via interaction with PARP1"
**作者**: Li et al. (2018)
**摘要**: 通过Western blot和免疫荧光(KIF4A抗体验证蛋白定位)，研究发现KIF4A通过结合PARP1调控DNA损伤修复通路，促进结直肠癌细胞对化疗药物耐药，靶向KIF4A可增强化疗敏感性。
3. **文献名称**: "KIF4A promotes hepatocellular carcinoma progression by activating the PI3K/AKT pathway"
**作者**: Wang et al. (2019)
**摘要**: 利用KIF4A抗体进行组织芯片分析，发现KIF4A在肝癌中高表达并与临床分期相关。机制研究表明，KIF4A通过激活PI3K/AKT信号通路促进肿瘤侵袭和转移。
4. **文献名称**: "Development and validation of a novel anti-KIF4A monoclonal antibody for clinical diagnostics"
**作者**: Chen et al. (2021)
**摘要**: 报道了一种高特异性KIF4A单克隆抗体的开发，通过ELISA、免疫印迹和流式细胞术验证其灵敏度及特异性，证实其在多种癌症组织中的诊断应用潜力。
(注：以上文献为示例，实际引用需以真实发表的论文为准。)
KIF4A (kinesin family member 4A) is a microtubule-associated motor protein belonging to the kinesin superfamily, which plays critical roles in intracellular transport, mitotic chromosome segregation, and cell division. It is involved in regulating microtubule dynamics during mitosis and meiosis, particularly in organizing the spindle apparatus and aligning chromosomes. KIF4A also participates in DNA repair, cell cycle progression, and maintaining genomic stability. Dysregulation of KIF4A has been linked to various cancers, including breast, lung, and colorectal cancers, where its overexpression often correlates with poor prognosis, tumor proliferation, and metastasis.
KIF4A antibodies are essential tools for studying its expression, localization, and functional mechanisms. They are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to detect KIF4A levels in cell lines, tissues, or clinical samples. Specific antibodies can differentiate between phosphorylated or isoform variants, aiding in research on post-translational modifications. Additionally, KIF4A antibodies are employed in co-immunoprecipitation studies to identify interacting partners, such as Aurora kinases or condensin complexes, shedding light on its role in mitosis and cancer pathways. Validated antibodies with high specificity are crucial for ensuring accurate experimental outcomes in both basic research and clinical applications.
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