| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | EBAF; LEFTA; LEFTB; LEFTY1; LEFTYA; LEFTYB; PSEC0024; TGFB4;;LEFTY |
| WB Predicted band size | Calculated MW: 41 kDa ; Observed MW: 34.44 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human LEFTY |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
+ +
以下是3篇涉及LEFTY1/LEFTY2抗体的代表性文献示例(注：以下为假设性文献框架，具体内容需根据实际文献调整)：
---
1. **文献名称**: *"Leftys asymmetry: roles of Lefty1/Lefty2 in embryonic left-right patterning"*
**作者**: Meno C. et al.
**摘要**: 研究LEFTY1/LEFTY2在小鼠胚胎左右轴形成中的作用，通过特异性抗体定位发现二者在左侧板中胚层和节点区域差异表达，调控Nodal信号通路。
2. **文献名称**: *"Lefty2 as a prognostic biomarker in breast cancer: an immunohistochemical study"*
**作者**: Sakuma R. et al.
**摘要**: 利用抗LEFTY2抗体对乳腺癌组织进行免疫组化分析，发现LEFTY2高表达与肿瘤转移抑制相关，提示其作为潜在预后标志物的价值。
3. **文献名称**: *"Antibody-based inhibition of LEFTY1/LEFTY2 enhances stem cell differentiation in vitro"*
**作者**: Chen Y. et al.
**摘要**: 通过抗LEFTY1/LEFTY2中和抗体阻断其功能，发现可促进人多能干细胞向心肌细胞分化，证实二者在维持干细胞多能性中的关键作用。
---
(注：以上文献为示例性质，实际引用需核查真实文献数据库如PubMed或Web of Science获取准确信息。)
LEFTY1 and LEFTY2. members of the TGF-β superfamily, are secreted cytokines critical in embryonic development, particularly in left-right axis patterning and mesoderm formation. They act as antagonists to Nodal signaling, regulating cell differentiation and tissue asymmetry. Beyond embryogenesis, these proteins are implicated in cancer progression, fibrosis, and immune modulation, often dysregulated in tumors or inflammatory conditions. Due to structural similarity and overlapping functions, distinguishing LEFTY1 and LEFTY2 via conventional methods is challenging.
Antibodies targeting LEFTY1+LEFTY2 are designed to detect both isoforms collectively, enabling researchers to study their combined roles in biological processes. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and ELISA to assess protein expression in developmental models, cancer studies, or regenerative medicine research. Their development addresses the need for tools to investigate redundant or synergistic functions of these paralogs, especially in contexts where isoform-specific antibodies may lack sensitivity.
Commercial LEFTY1+LEFTY2 antibodies are typically validated for cross-reactivity across species (e.g., human, mouse) and tested for specificity against related TGF-β proteins. Challenges include minimizing off-target binding while maintaining affinity for conserved epitopes. Such reagents are pivotal in exploring therapeutic strategies targeting LEFTY signaling, including cancer metastasis inhibition or tissue repair modulation. Ongoing research leverages these antibodies to decipher their roles in stem cell niches and tumor microenvironments, highlighting their dual diagnostic and functional significance.
×
