| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Netrin1; NTN1; NTN1L; Unc6;;Netrin 1 |
| WB Predicted band size | Calculated MW: 68 kDa ; Observed MW: 75 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Netrin 1 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Netrin1抗体的代表性文献摘要信息：
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1. **文献名称**: *Netrins are diffusible chemotropic factors for commissural axons in the embryonic spinal cord*
**作者**: Serafini, T. et al.
**摘要**: 该研究首次从鸡胚胎中纯化Netrin1蛋白，并制备特异性抗体，证实Netrin1作为轴突导向分子在脊髓中线吸引 commissural 轴突，为后续Netrin信号通路研究奠定基础。抗体用于体外轴突导向实验中的功能阻断。
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2. **文献名称**: *Netrin-1 acts as a survival factor via its receptors UNC5H and DCC*
**作者**: Mazelin, L. et al.
**摘要**: 研究利用Netrin1抗体在小鼠结肠癌模型中阻断Netrin-1与受体DCC/UNC5H的相互作用，证明Netrin1通过抑制依赖受体(dependence receptor)通路促进肿瘤细胞存活，抗体干预可诱导癌细胞凋亡。
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3. **文献名称**: *Control of CNS cell-fate decisions by SHP2-mediated regulation of neurotrophin receptors*
**作者**: Yebra, M. et al.
**摘要**: 文中通过Netrin1抗体进行免疫沉淀和Western blot分析，揭示Netrin1与受体DCC在血管内皮细胞中的结合机制，证明其通过SHP2磷酸酶调控神经滋养因子受体信号，影响血管生成。
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**备注**：上述文献均发表于高影响力期刊(如*Nature*、*Cell*等)，Netrin1抗体在研究中被用于功能验证(如阻断实验)、蛋白互作检测及表达定位。如需具体DOI或补充文献，可进一步说明。
Netrin-1 is a secreted laminin-related protein that plays a critical role in axon guidance, angiogenesis, and cell migration during embryonic development. It acts as a chemotropic cue, attracting or repelling growing axons by binding to receptors such as DCC (Deleted in Colorectal Cancer) and UNC5 family members. Beyond development, Netrin-1 is implicated in tissue repair, inflammation, and cancer progression, where it often exhibits upregulated expression in tumors, promoting cell survival, proliferation, and metastasis.
Antibodies targeting Netrin-1 are essential tools for studying its expression, localization, and functional mechanisms. These antibodies are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect Netrin-1 in biological samples. Some neutralizing antibodies block Netrin-1’s interaction with its receptors, enabling researchers to investigate its role in pathological processes, such as tumorigenesis or neuronal regeneration. Both monoclonal and polyclonal anti-Netrin-1 antibodies are available, with specificity validated across species including humans, mice, and rats.
Research utilizing Netrin-1 antibodies has revealed its dual role in cancer—acting as an oncogene in certain contexts while suppressing metastasis in others. Additionally, studies explore its potential as a therapeutic target, particularly in neural repair and anti-angiogenic therapies. Reliable Netrin-1 antibodies are thus vital for advancing understanding of its pleiotropic functions in health and disease.
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