| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BUB3; BUB 3L;;BUB3 |
| WB Predicted band size | Calculated MW: 37 kDa ; Observed MW: 40 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human BUB3 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于Bub3抗体的3篇参考文献(基于公开信息概括，非真实文献，仅供参考)：
1. **文献名称**：*Bub3 is a spindle assembly checkpoint protein regulating chromosome segregation*
**作者**：Taylor, S.S., et al.
**摘要**：该研究利用Bub3特异性抗体，通过免疫沉淀和免疫荧光技术，揭示了Bub3在有丝分裂检查点中的核心作用，证明其与Bub1/BubR1的相互作用对染色体正确分离至关重要。
2. **文献名称**：*Mechanistic insights into the spindle checkpoint through Bub3 antibody-based functional assays*
**作者**：Lara-Gonzalez, P., et al.
**摘要**：通过开发高特异性Bub3抗体，作者解析了Bub3在检查点信号传导中的构象变化，并发现其缺失导致癌细胞对微管抑制剂敏感性增加，为靶向治疗提供依据。
3. **文献名称**：*Bub3 antibody reveals dysregulation in human cancers with chromosomal instability*
**作者**：Zhang, Y., et al.
**摘要**：研究利用Bub3抗体对多种癌症组织进行染色分析，发现Bub3表达水平与染色体不稳定性呈正相关，提示其可作为癌症预后标志物的潜在价值。
(注：以上文献为示例性质，实际引用请通过PubMed或学术数据库检索真实发表论文。)
Bub3 is a critical component of the spindle assembly checkpoint (SAC), a surveillance mechanism ensuring accurate chromosome segregation during mitosis. It forms a complex with other SAC proteins, such as Bub1 and BubR1. to monitor kinetochore-microtubule attachment and delay anaphase until all chromosomes are properly aligned. Dysregulation of Bub3 is linked to chromosomal instability, a hallmark of many cancers, and defects in SAC function may contribute to tumorigenesis or therapeutic resistance.
Bub3 antibodies are essential tools for studying mitotic regulation, genome stability, and cancer biology. They are widely used in techniques like Western blotting, immunofluorescence, and immunoprecipitation to detect Bub3 expression levels, localization, and protein interactions. Researchers employ these antibodies to investigate Bub3's role in SAC signaling, its interplay with other checkpoint components, and its potential as a biomarker in malignancies. Commercial Bub3 antibodies are typically developed using immunogens derived from conserved regions of human Bub3. ensuring cross-reactivity with orthologs in model organisms. Validation steps, including knockout cell line controls, are crucial to confirm specificity.
In cancer research, Bub3 antibodies help elucidate mechanisms behind chemotherapy resistance driven by SAC hyperactivation or identify tumors with SAC deficiencies. Their applications extend to studying developmental disorders and exploring therapeutic strategies targeting mitotic checkpoints.
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