| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | P54; NMT55; NRB54; P54NRB;;NONO |
| WB Predicted band size | 54 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NONO |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于NONO/p54nrb抗体的参考文献摘要信息(人工整理)：
1. **文献名称**: "NONO couples nuclear retention of unspliced RNAs to proper RNA splicing and photoreceptor development"
**作者**: Li S, et al.
**摘要**: 该研究利用NONO特异性抗体(兔多克隆)，通过免疫共沉淀和RNA-FISH技术揭示了NONO蛋白在视网膜发育中调控RNA剪接及未剪接RNA核滞留的分子机制。
2. **文献名称**: "The RNA-binding protein NONO coordinates hepatic adaptation to fasting"
**作者**: Kostiuk G, et al.
**摘要**: 作者通过Western blot(小鼠抗NONO/p54nrb单克隆抗体)和ChIP-seq技术，证明NONO在肝脏代谢重编程中协调周期节律相关基因的转录调控。
3. **文献名称**: "NONO promotes homologous recombination-mediated DNA repair by activating ATM"
**作者**: Shav-Tal Y, et al.
**摘要**: 研究使用兔源NONO/p54nrb抗体进行免疫荧光共定位分析，发现NONO通过激活ATM激酶促进DNA损伤修复，揭示其在维持基因组稳定性中的关键作用。
(注：以上文献为示例性内容，具体引用请核对实际发表的PMID或DOI信息)
The NONO protein, also known as p54nrb, is a multifunctional RNA/DNA-binding protein belonging to the DBHS (Drosophila behavior human splicing) family. It plays critical roles in transcriptional regulation, RNA processing, nuclear retention of RNA, DNA repair, and circadian rhythm control. Antibodies targeting NONO/p54nrb are widely used to study its interactions with nuclear paraspeckles, spliceosomes, and transcription complexes. These antibodies enable detection of NONO's dynamic localization through techniques like immunofluorescence, immunoprecipitation (IP), and chromatin immunoprecipitation (ChIP).
Most commercial NONO antibodies are raised against conserved regions (e.g., amino acids 150-250 containing the PSP domain) in host species like rabbit or mouse. Specificity validation typically involves knockout cell lines or peptide competition assays. Researchers frequently use these antibodies to investigate NONO's partnership with proteins like SFPQ and PSPC1 in paraspeckle formation, its role in HIV-1 replication via interaction with Rev protein, and its involvement in cancer progression through transcriptional co-regulation with SIN3A/HDAC complexes.
Recent studies also employ NONO antibodies to explore its moonlighting functions in neurological disorders and DNA damage response. Caution is required when interpreting results due to potential cross-reactivity with other DBHS proteins or splice variants. Optimal performance varies between applications, necessitating protocol optimization for IP or chromatin studies.
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