| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/20-1/100 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | CDC21; CDC54; hCdc21; P1-CDC21;;MCM4 |
| WB Predicted band size | 97 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human MCM4 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于MCM4抗体的参考文献示例(注：文献信息为模拟生成，仅供参考)：
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1. **标题**：*"MCM4 as a marker of DNA replication licensing in human cells"*
**作者**：Ishimi Y. et al.
**摘要**：本研究利用MCM4特异性抗体，通过免疫沉淀和Western blot技术，揭示了MCM4在DNA复制起始中的关键作用。实验表明，MCM4与其他MCM家族蛋白形成复合体，调控细胞周期中复制起点的许可过程。
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2. **标题**：*"Overexpression of MCM4 in colorectal cancer and its clinical implications"*
**作者**：Taneja P. et al.
**摘要**：通过免疫组化分析，使用MCM4抗体检测结直肠癌组织样本，发现MCM4表达水平与肿瘤分级和患者预后显著相关，提示其作为潜在癌症生物标志物的价值。
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3. **标题**：*"A novel MCM4 mutation causes primary ovarian insufficiency in a consanguineous family"*
**作者**：Perveen R. et al.
**摘要**：本研究通过全外显子测序发现MCM4基因突变与卵巢早衰相关，利用MCM4抗体进行蛋白质表达分析，证实突变导致MCM复合体功能异常，影响生殖细胞发育。
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**注**：以上文献为示例，实际引用时请核实真实文献信息(可通过PubMed、Google Scholar等平台搜索"MCM4 antibody" + 研究关键词)。
**Background of MCM4 Antibody**
The minichromosome maintenance complex component 4 (MCM4) is a critical protein involved in DNA replication initiation and elongation. As a member of the MCM2-7 hexameric helicase complex, MCM4 plays a vital role in unwinding DNA during the S phase of the cell cycle, ensuring faithful genome duplication. Dysregulation of MCM proteins is linked to replication stress, genomic instability, and cancer progression.
MCM4 antibodies are essential tools for studying DNA replication mechanisms, cell cycle regulation, and cancer biology. These antibodies specifically detect MCM4 expression in various experimental applications, including Western blotting, immunofluorescence, and immunohistochemistry. Elevated MCM4 levels are observed in proliferating cells, making it a biomarker for cellular proliferation and malignancy. Research has also implicated MCM4 mutations in genetic disorders, such as natural killer cell deficiency and adrenal gland dysfunction.
In cancer diagnostics, MCM4 antibodies help assess tumor aggressiveness and replication stress responses. Additionally, studies explore MCM4’s interaction with licensing factors (e.g., Cdc6. Cdt1) and checkpoint regulators (e.g., ATR, Chk1), providing insights into replication fork stability and therapeutic targeting. Overall, MCM4 antibodies are pivotal in unraveling replication-related pathologies and developing strategies to combat cancer and genetic diseases.
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