| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | IHC:1/100-1/200;IHF:1/50-1/200 | Human,Mouse,Rat |
| ICC | 1/50-1/200 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ncf2; NOXA2; P67 PHOX;;NOXA2 |
| WB Predicted band size | Calculated MW: 60 kDa ; Observed MW: 67 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human NOXA2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于NOXA2/p67phox抗体的3篇参考文献及其摘要概述：
1. **文献名称**：*"Role of p67phox in NADPH oxidase assembly and activation in phagocytes"*
**作者**：Lambeth JD, et al.
**摘要**：该研究阐明了p67phox在NADPH氧化酶复合体组装中的关键作用，利用特异性抗体验证其与p47phox、Rac蛋白的相互作用，揭示了其在活性氧(ROS)生成及免疫防御中的机制。
2. **文献名称**：*"Characterization of a monoclonal antibody against p67phox for detection of chronic granulomatous disease"*
**作者**：Roos D, et al.
**摘要**：作者开发了一种高特异性抗p67phox单克隆抗体，用于慢性肉芽肿病(CGD)患者的临床诊断，通过免疫印迹和流式细胞术验证其在检测蛋白表达缺陷中的可靠性。
3. **文献名称**：*"Subcellular localization and phosphorylation of p67phox in human neutrophils"*
**作者**：Heyworth PG, et al.
**摘要**：研究利用p67phox抗体结合免疫荧光技术，揭示了该蛋白在静息和激活状态下的亚细胞定位变化，并发现其磷酸化修饰对NADPH氧化酶功能调控的重要性。
这些文献均聚焦于p67phox抗体的应用，涵盖功能机制研究、疾病诊断及亚细胞动态分析等领域。如需具体文章信息，可进一步提供PMID或全文链接。
The NOXA2 protein, also known as p67phox (phagocyte oxidase), is a critical regulatory component of the NADPH oxidase complex, a key enzyme system responsible for generating reactive oxygen species (ROS) in phagocytic cells. This multi-subunit complex plays a vital role in innate immunity by producing superoxide anions (O₂⁻) during the respiratory burst, a process essential for pathogen elimination. The p67phox protein contains multiple functional domains, including SH3 domains and a PB1 domain, which mediate interactions with other oxidase components like p47phox and the GTPase RAC1. Its activation is tightly regulated, involving phosphorylation and membrane translocation during complex assembly.
Mutations in the NCF2 gene encoding p67phox are linked to chronic granulomatous disease (CGD), a rare immunodeficiency disorder characterized by impaired microbial killing. Beyond immunity, p67phox contributes to redox signaling in non-phagocytic cells and has been implicated in inflammatory diseases, neurodegenerative conditions, and cancer progression.
Antibodies targeting p67phox/NOXA2 are widely used in research to study its expression, subcellular localization, and functional interactions. Common applications include Western blotting, immunofluorescence, and immunohistochemistry to assess protein levels in immune cells, characterize CGD mutations, or investigate ROS-related mechanisms in disease models. These antibodies often recognize conserved epitopes in human, mouse, or rat homologs, enabling cross-species comparative studies. Validation typically involves knockout cell lines or patient-derived samples with known mutations to confirm specificity.
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