| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 1/20-1/50 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Myf5; Class C basic helix loop helix protein 2;MYF5 |
| WB Predicted band size | Calculated MW: 28 kDa ; Observed MW: 37 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human MYF5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于MYF5抗体的参考文献(均为虚构示例，仅用于演示格式)：
1. **文献名称**: "MYF5 expression in embryonic skeletal muscle development"
**作者**: Smith J, et al.
**摘要**: 研究利用MYF5抗体通过免疫组化技术追踪小鼠胚胎期骨骼肌前体细胞的分化过程，证实MYF5在成肌细胞定向分化中的早期调控作用。
2. **文献名称**: "Comparative analysis of MYF5 antibody specificity across species"
**作者**: Lee H, et al.
**摘要**: 评估了多种MYF5抗体在人类、小鼠和斑马鱼模型中的交叉反应性，发现抗体Clone 2B9在跨物种标记中具有高特异性和稳定性。
3. **文献名称**: "MYF5 as a biomarker for rhabdomyosarcoma diagnosis"
**作者**: Garcia R, et al.
**摘要**: 通过MYF5抗体免疫染色发现，MYF5在横纹肌肉瘤组织中显著高表达，提示其可作为该恶性肿瘤的潜在诊断标志物。
(注：以上文献信息为模拟生成，如需真实文献，请通过PubMed或Google Scholar检索关键词“MYF5 antibody”获取。)
MYF5 (myogenic factor 5) is a transcription factor critical for skeletal muscle development and regeneration. As a member of the myogenic regulatory factor (MRF) family, it works alongside MYOD, MYF6. and MYOG to orchestrate myoblast specification, proliferation, and differentiation during embryogenesis. MYF5 is notably expressed in early myogenic progenitor cells and satellite cells, serving as a key marker for muscle stem cell populations. Antibodies targeting MYF5 are widely used in developmental biology and muscle-related research to identify and track myogenic lineages, assess muscle regeneration processes, and study congenital myopathies.
These antibodies are essential tools in techniques like immunohistochemistry, Western blotting, and flow cytometry, enabling visualization of MYF5 expression patterns in tissues or cultured cells. Commercially available MYF5 antibodies are typically validated for specificity across human, mouse, and rat models, with recombinant protein controls often used to confirm binding accuracy. Researchers also employ them in disease models, such as muscular dystrophies or sarcopenia, to evaluate therapeutic interventions targeting muscle repair. Recent studies further explore MYF5's potential role in rhabdomyosarcoma and its utility in differentiating pluripotent stem cells into myogenic lineages for regenerative medicine applications.
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