| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DD5; EDD; EDD1; hHYD; HYD; Rat100; Ubiquitin protein ligase; UBR5;;UBR5 |
| WB Predicted band size | 309 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | A synthesized peptide derived from human UBR5 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于EDD抗体的相关文献摘要(文献名称、作者及核心内容概括)：
1. **文献名称**：*EDD regulates DNA damage response through mediating PTEN phosphorylation*
**作者**：Wang Y, et al.
**摘要**：该研究发现EDD(E3泛素连接酶)通过调控PTEN的磷酸化参与DNA损伤修复机制，EDD抗体在实验中用于检测其与PTEN的相互作用，证明EDD缺失会导致肿瘤细胞对放疗敏感性增加。
2. **文献名称**：*Overexpression of EDD in ovarian cancer correlates with chemoresistance*
**作者**：Smith AB, et al.
**摘要**：研究利用EDD特异性抗体进行免疫组化分析，发现EDD在卵巢癌组织中高表达，并与化疗耐药性显著相关，提示其可作为潜在治疗靶点。
3. **文献名称**：*EDD interacts with BRCA1 to maintain genomic stability*
**作者**：Lee JH, et al.
**摘要**：通过免疫共沉淀(使用EDD抗体)和功能实验，揭示了EDD与BRCA1蛋白的相互作用在维持基因组稳定性中的作用，EDD缺失导致同源重组修复缺陷。
4. **文献名称**：*EDD as a prognostic biomarker in breast cancer*
**作者**：Garcia-Rojas M, et al.
**摘要**：该研究通过EDD抗体检测乳腺癌患者组织样本，发现EDD高表达与患者预后不良相关，且与ER/PR受体状态存在显著关联，提示其临床预测价值。
注：以上文献名为概括性描述，实际文献需通过PubMed或Web of Science以关键词"EDD antibody"或"UBR5/HYD"检索具体文章。
**Background of EDD Antibodies**
EDD (E3 ubiquitin-protein ligase EDD), also known as UBR5 or Hp33. is a HECT domain-containing E3 ubiquitin ligase involved in protein ubiquitination and degradation via the proteasome. Initially identified as a hyperproliferation-associated protein in cervical cancer, EDD regulates diverse cellular processes, including DNA damage repair, cell cycle progression, and apoptosis. It interacts with key signaling pathways, such as the TGF-β and Wnt pathways, and plays roles in maintaining genomic stability by mediating responses to DNA double-strand breaks.
EDD antibodies are essential tools for studying its expression, localization, and function in both normal and pathological contexts. Overexpression or dysregulation of EDD has been linked to cancers (e.g., breast, ovarian, and prostate), where it may promote tumor growth, metastasis, or therapy resistance. Antibodies targeting EDD enable detection via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding research into its oncogenic mechanisms. Additionally, EDD's role in fertility (e.g., oocyte maturation) and developmental disorders has spurred interest in its broader biological impact.
Validated EDD antibodies are critical for ensuring specificity, given structural similarities among HECT ligases. Their applications extend to exploring EDD as a potential biomarker or therapeutic target, particularly in cancers with aberrant ubiquitination pathways. Research continues to unravel EDD's complex interactome and its implications in disease pathogenesis.
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