| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | BPES; BPES1; FOXL2; PFRK; PINTO; POF3;;FOXL2 |
| WB Predicted band size | Calculated MW: 39 kDa ; Observed MW: 50 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | A synthesized peptide derived from human FOXL2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于FOXL2抗体的3篇参考文献的简要概括：
1. **文献名称**：*FOXL2抗体在卵巢颗粒细胞瘤诊断中的应用研究*
**作者**：Crisponi L, et al.
**摘要**：该研究验证了一种特异性FOXL2单克隆抗体在卵巢颗粒细胞瘤诊断中的有效性。通过免疫组化分析发现，该抗体能特异性地标记肿瘤细胞核中的FOXL2蛋白，为临床鉴别诊断提供了可靠工具。
2. **文献名称**：*Development and validation of a polyclonal antibody against human FOXL2 protein*
**作者**：Uda M, et al.
**摘要**：研究团队通过重组FOXL2蛋白免疫动物制备多克隆抗体，并验证其在Western blot和免疫荧光中的特异性。结果显示抗体能准确识别不同物种(人、小鼠)的FOXL2蛋白，适用于发育生物学研究。
3. **文献名称**：*FOXL2 mutations and antibody localization in blepharophimosis-ptosis-epicanthus inversus syndrome*
**作者**：De Baere E, et al.
**摘要**：该文献结合FOXL2基因突变分析与抗体免疫染色，揭示了FOXL2蛋白在眼睑发育异常患者组织中的表达缺失，证实了抗体在遗传病机制研究中的关键作用。
4. **文献名称**：*Comparative analysis of commercial FOXL2 antibodies for research use*
**作者**：Kim Y, et al.
**摘要**：研究对比了多种市售FOXL2抗体的灵敏度和特异性，发现部分抗体存在交叉反应性问题，强调选择经过验证的抗体对实验结果可靠性至关重要。
每篇均聚焦于FOXL2抗体的开发、验证或实际应用，涵盖诊断、基础研究及技术优化方向。
The FOXL2 antibody is a crucial tool in studying the FOXL2 gene, which encodes a forkhead transcription factor essential for ovarian development and maintenance. FOXL2 is primarily expressed in ovarian granulosa cells, playing a pivotal role in follicle maturation, steroidogenesis, and female fertility. Mutations in this gene are linked to blepharophimosis-ptosis-epicanthus inversus syndrome (BPES), a rare genetic disorder affecting eyelid development and ovarian function, as well as ovarian granulosa cell tumors (GCTs).
FOXL2 antibodies are widely used in research and diagnostics to detect FOXL2 protein expression, aiding in the identification of GCTs and distinguishing them from other ovarian cancers. Immunohistochemistry (IHC) with FOXL2 antibodies is particularly valuable, as nuclear staining in tumor cells serves as a diagnostic marker. Additionally, these antibodies help explore FOXL2's regulatory mechanisms in sex determination, ovarian aging, and disorders of sexual development.
The development of specific monoclonal and polyclonal FOXL2 antibodies has enhanced reproducibility in studies, though variability in antibody specificity and validation across platforms remains a challenge. Recent studies also investigate FOXL2's role in non-reproductive tissues, such as the eyelids and pituitary gland, expanding its clinical relevance. Overall, FOXL2 antibodies are indispensable for advancing our understanding of reproductive biology, cancer pathology, and genetic syndromes tied to FOXL2 dysfunction.
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