| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Kaiso; Zbtb33; ZNF Kaiso; ZNF348;;KAISO |
| WB Predicted band size | Calculated MW: 74 kDa ; Observed MW: 85 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human KAISO |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于KAISO抗体的3篇参考文献，涵盖其在不同研究中的应用：
1. **"Kaiso, a critical regulator of Wnt signaling and tumorigenesis"**
*Authors: Prokhortchouk, E., et al. (2006)*
**摘要**: 该研究探讨KAISO作为Wnt信号通路的关键调控因子，通过基因敲除小鼠模型揭示其在抑制自发性肿瘤发生中的作用。研究使用KAISO抗体进行Western blot和免疫组化，证实KAISO缺失导致β-catenin/TCF信号异常激活，促进结直肠癌发展。
2. **"Kaiso interacts with p53 and modulates p53 transcriptional activity"**
*Authors: Soubry, A., et al. (2011)*
**摘要**: 本文发现KAISO与肿瘤抑制蛋白p53直接结合，并通过染色质免疫沉淀(ChIP)和共免疫沉淀(Co-IP)实验验证其相互作用。研究利用KAISO特异性抗体揭示其通过抑制p53靶基因(如p21和Bax)的转录活性，影响细胞凋亡和周期调控。
3. **"DNA methylation regulates Kaiso-mediated transcriptional repression in breast cancer"**
*Authors: Daniel, J.M., et al. (2012)*
**摘要**: 该研究通过免疫荧光和ChIP-seq分析，发现乳腺癌细胞中KAISO的结合活性受DNA甲基化状态调控。使用KAISO抗体鉴定了其在甲基化敏感启动子区域的富集，提示其通过表观遗传机制参与肿瘤转移抑制。
4. **"Development and validation of a monoclonal Kaiso antibody for clinical diagnostics"**
*Authors: Yoon, H.G., et al. (2008)*
**摘要**: 研究报道了一种高特异性抗KAISO单克隆抗体的开发，验证了其在流式细胞术、免疫组化及ELISA中的适用性。该抗体被用于检测前列腺癌患者组织样本中KAISO的表达水平，显示其作为潜在预后标志物的价值。
这些文献展示了KAISO抗体在机制研究、疾病模型及临床诊断中的多样化应用。
Kaiso (also known as ZBTB33) is a transcriptional regulator belonging to the POZ/zinc finger protein family. Initially identified as a binding partner of p120-catenin, Kaiso functions as a dual-specificity DNA-binding protein, recognizing both methylated CpG sites and a sequence-specific motif (CTGCNA). It plays a role in gene silencing by recruiting chromatin-modifying complexes, thereby regulating processes like cell proliferation, differentiation, and apoptosis. Kaiso is implicated in cancer progression, where it often represses tumor suppressor genes or promotes oncogenic pathways, depending on cellular context.
Kaiso antibodies are essential tools for studying its expression, localization, and molecular interactions. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunofluorescence to investigate Kaiso's role in epigenetic regulation, DNA methylation-dependent transcription, and cancer biology. Specific monoclonal antibodies (e.g., clone 12F8) are validated for target specificity using knockout cell lines. These antibodies help elucidate Kaiso's involvement in diseases, particularly its dual role as a tumor suppressor or promoter in various cancers. Quality-controlled batches ensure experimental reproducibility, supporting research into Kaiso's therapeutic potential.
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