| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | USP28 ;;USP28 |
| WB Predicted band size | Calculated MW: 122 kDa ; Observed MW: 135 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human USP28 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是关于USP28抗体的3篇参考文献及其摘要概括：
1. **"USP28 regulates MYC stability through TRAF6-mediated ubiquitination"**
*作者：Pópulo H, et al.*
摘要：研究揭示了USP28通过去泛素化稳定c-MYC蛋白的机制，利用USP28抗体进行Western blot验证其在癌细胞中的表达及功能，提示USP28作为潜在癌症治疗靶点。
2. **"USP28 interacts with 53BP1 and promotes DNA repair through deubiquitination"**
*作者：Zhang Y, et al.*
摘要：通过免疫共沉淀(IP)和免疫荧光实验，证明USP28抗体可用于检测其与DNA损伤修复蛋白53BP1的相互作用，USP28通过调控泛素化修复双链断裂。
3. **"The deubiquitinase USP28 controls oncogenic cellular programs through multiple substrates"**
*作者：Schülein-Völk C, et al.*
摘要：研究利用USP28抗体进行组织芯片(IHC)分析，发现其在多种肿瘤中高表达，并通过去泛素化靶蛋白(如c-JUN)驱动肿瘤发生，强调其底物广泛性。
4. **"USP28 antagonizes FBW7 to sustain NOTCH stability in T-cell leukemia"**
*作者：Mavrakis KJ, et al.*
摘要：使用USP28抗体验证其在白血病细胞中与FBW7的竞争关系，表明USP28通过稳定NOTCH蛋白促进T细胞恶性转化，为靶向治疗提供依据。
这些研究均通过USP28抗体进行蛋白功能验证，涵盖癌症、DNA修复及信号通路调控等领域。
The ubiquitin-specific protease 28 (USP28) is a deubiquitinating enzyme involved in regulating critical cellular processes, including DNA damage response, cell cycle progression, and apoptosis. It functions by removing ubiquitin chains from target proteins, counteracting ubiquitin-mediated degradation, and stabilizing key regulators such as c-MYC, p53. and Claspin. USP28 is notably associated with the Fanconi anemia pathway and plays a role in maintaining genomic stability. Dysregulation of USP28 has been implicated in cancer development, as it can promote tumorigenesis by stabilizing oncoproteins or inhibiting tumor suppressors.
USP28 antibodies are essential tools for studying its expression, localization, and function in various biological contexts. These antibodies are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to detect USP28 protein levels in cell lines or tissue samples. Researchers also employ them to investigate USP28's interaction partners and its role in cellular stress responses or cancer pathways. Due to its therapeutic potential, USP28 is explored as a target for small-molecule inhibitors, and specific antibodies aid in validating target engagement in preclinical studies. Commercial USP28 antibodies are typically validated for specificity and sensitivity across human and model organism samples.
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