| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Atoh4; bHLHa8; Math4A; MGC; NEUROG2; Neurogenin2; NGN2;;Neurogenin 2 |
| WB Predicted band size | Calculated MW: 29 kDa ; Observed MW: 25 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | A synthesized peptide derived from human Neurogenin 2 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.05% BSA and 50% glycerol. |
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以下是3篇关于Neurogenin2(Ngn2)抗体的参考文献摘要：
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1. **文献名称**: "Neurogenin2 regulates progenitor cell-cycle progression and basal motility in the cerebral cortex"
**作者**: Lacomme M et al.
**摘要**: 本研究利用Neurogenin2抗体进行免疫组化和Western blot分析，揭示了Ngn2在大脑皮层神经前体细胞周期调控和运动性中的作用，发现其通过调控Cyclin D1和p27Kip1影响神经发生进程。
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2. **文献名称**: "Direct conversion of fibroblasts to functional neurons by defined factors"
**作者**: Vierbuchen T et al.
**摘要**: 通过Neurogenin2抗体验证诱导神经元重编程过程中Ngn2的表达，证实其与Ascl1、Myt1l共同作用可将成纤维细胞直接转化为功能性神经元，为神经再生研究提供新策略。
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3. **文献名称**: "Specification of dorsal telencephalic derivatives by the bHLH transcription factor Neurogenin2"
**作者**: Schuurmans C et al.
**摘要**: 研究采用Ngn2特异性抗体进行原位杂交和免疫荧光，证明Ngn2在端脑背侧区域神经干细胞分化中的关键作用，调控皮层神经元亚型特化和迁移过程。
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4. **文献名称**: "Neurogenin2 expression defines transitional生长 zones in the developing mouse nervous system"
**作者**: Gradwohl G et al.
**摘要**: 通过Ngn2抗体的免疫染色，揭示了小鼠胚胎神经系统中Ngn2在神经嵴细胞和感觉神经节中的动态表达模式，提示其参与感觉神经元和自主神经系统的发育调控。
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以上文献均通过Neurogenin2抗体进行蛋白表达验证或功能研究，涉及神经发生、细胞重编程及发育调控等领域。
Neurogenin2 (Ngn2) is a basic helix-loop-helix (bHLH) transcription factor critical in regulating neurogenesis during embryonic development. It plays a pivotal role in specifying neuronal cell fate, particularly in the central nervous system, by promoting the differentiation of neural progenitor cells into neurons while suppressing glial cell lineages. Ngn2 is transiently expressed during early neuronal commitment, making it a key marker for studying neurodevelopmental processes, neuronal differentiation, and cell lineage determination.
Antibodies targeting Neurogenin2 are essential tools for detecting and visualizing Ngn2 protein expression in various experimental models, including immunohistochemistry, Western blotting, and immunofluorescence. These antibodies enable researchers to map Ngn2's spatiotemporal expression patterns in tissues, assess its role in neuronal differentiation, and investigate its dysregulation in neurodevelopmental disorders or diseases. Specificity and validation (e.g., knockout controls) are critical for reliable results, as Ngn2 shares structural homology with other bHLH family members like Neurogenin1 and NeuroD.
Recent applications include stem cell research, where Ngn2 overexpression is used to reprogram fibroblasts or induced pluripotent stem cells (iPSCs) into functional neurons. Commercially available Ngn2 antibodies are often raised against conserved epitopes in human, mouse, or rat proteins, with validation in model organisms like zebrafish or chick embryos. Proper controls and species reactivity verification remain essential for experimental accuracy.
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