| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SUMF1 |
| Uniprot No | Q8NBK3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 34-374aa |
| 氨基酸序列 | SQEAGTGAGAGSLAGSCGCGTPQRPGAHGSSAAAHRYSREANAPGPVPGE RQLAHSKMVPIPAGVFTMGTDDPQIKQDGEAPARRVTIDAFYMDAYEVSN TEFEKFVNSTGYLTEAEKFGDSFVFEGMLSEQVKTNIQQAVAAAPWWLPV KGANWRHPEGPDSTILHRPDHPVLHVSWNDAVAYCTWAGKRLPTEAEWEY SCRGGLHNRLFPWGNKLQPKGQHYANIWQGEFPVTNTGEDGFQGTAPVDA FPPNGYGLYNIVGNAWEWTSDWWTVHHSVEETLNPKGPPSGKDRVKKGGS YMCHRSYCYRYRCAARSQNTPDSSASNLGFRCAADRLPTMDVHHHHHH |
| 预测分子量 | 38 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SUMF1重组蛋白的3篇示例参考文献(内容为模拟概括，非真实文献)：
1. **文献名称**: *Functional characterization of recombinant human SUMF1: a critical factor for sulfatase activation*
**作者**: Dierks T, et al.
**摘要**: 研究通过大肠杆菌表达系统成功纯化重组人SUMF1蛋白，证实其通过氧化修饰硫酸酯酶活性位点的半胱氨酸为Cα-甲酰甘氨酸(FGly)，从而激活多种硫酸酯酶的催化功能。
2. **文献名称**: *Structural insights into SUMF1-mediated sulfatase maturation*
**作者**: Fraldi A, et al.
**摘要**: 利用X射线晶体学解析了重组SUMF1蛋白的三维结构，揭示了其与硫酸酯酶结合的活性域，阐明了其催化FGly生成的分子机制，为治疗多发性硫酸酯酶缺乏症提供结构基础。
3. **文献名称**: *Recombinant SUMF1 enhances sulfatase activity in a cellular model of multiple sulfatase deficiency*
**作者**: Cosma MP, et al.
**摘要**: 通过哺乳动物细胞表达重组SUMF1蛋白，证明其能在患者来源的成纤维细胞中恢复多种硫酸酯酶的活性，提示基因治疗或酶替代疗法的潜在应用价值。
(注：以上为基于SUMF1已知功能的模拟文献，实际引用请查询PubMed或相关数据库获取真实论文。)
**Background of SUMF1 Recombinant Protein**
SUMF1 (sulfatase modifying factor 1) is a critical enzyme responsible for the post-translational activation of sulfatases, a family of enzymes that hydrolyze sulfate esters. These sulfatases play essential roles in cellular processes, including the degradation of glycosaminoglycans (GAGs), steroid sulfates, and other sulfated molecules. SUMF1 catalyzes the oxidation of a conserved cysteine residue within sulfatases to formylglycine (FGly), a modification necessary for their catalytic activity. Defects in SUMF1 are linked to multiple sulfatase deficiency (MSD), a rare autosomal recessive lysosomal storage disorder characterized by impaired sulfatase function, leading to systemic accumulation of substrates and severe multisystemic pathology.
Recombinant SUMF1 protein is produced using biotechnological platforms, such as bacterial or mammalian expression systems, to enable large-scale production for research and therapeutic applications. Its recombinant form retains the ability to activate sulfatases *in vitro* and *in vivo*, making it valuable for studying sulfatase biology, developing enzyme replacement therapies (ERTs), or gene therapies for MSD and related disorders. Additionally, SUMF1 recombinant protein is utilized in drug screening assays to identify modulators of sulfatase activity.
Research on SUMF1 also explores its potential beyond sulfatase activation, including roles in cancer, inflammation, and neurodegenerative diseases, where sulfatase dysregulation may contribute to pathogenesis. The development of recombinant SUMF1 underscores its significance as a molecular tool and therapeutic candidate in addressing sulfatase-associated disorders.
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