| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SULT2B1 |
| Uniprot No | O00204 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311aa |
| 氨基酸序列 | MDGPAEPQIPGLWDTYEDDISEISQKLPGEYFRYKGVPFPVGLYSLESIS LAENTQDVRDDDIFIITYPKSGTTWMIEIICLILKEGDPSWIRSVPIWER APWCETIVGAFSLPDQYSPRLMSSHLPIQIFTKAFFSSKAKVIYMGRNPR DVVVSLYHYSKIAGQLKDPGTPDQFLRDFLKGEVQFGSWFDHIKGWLRMK GKDNFLFITYEELQQDLQGSVERICGFLGRPLGKEALGSVVAHSTFSAMK ANTMSNYTLLPPSLLDHRRGAFLRKGVCGDWKNHFTVAQSEAFDRAYRKQ MRGMPTFPWDEVDHHHHHH |
| 预测分子量 | 37 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SULT2B1重组蛋白的3篇参考文献概览：
1. **文献名称**: "Molecular cloning and characterization of a novel sulfotransferase, SULT2B1. in human prostate"
**作者**: Fuda, H., et al.
**摘要**: 该研究首次克隆并鉴定了人SULT2B1重组蛋白，证实其在胆固醇硫酸化中的特异性活性，并发现其在前列腺组织中高表达，提示其在类固醇代谢中的潜在作用。
2. **文献名称**: "Functional characterization of human sulfotransferase 2B1b (SULT2B1b) in recombinant expression systems"
**作者**: He, D., et al.
**摘要**: 通过在大肠杆菌及昆虫细胞中重组表达SULT2B1b，系统分析了其酶动力学参数及底物选择性，发现其对胆固醇衍生物具有高亲和力，并揭示了pH和离子环境对其活性的调控机制。
3. **文献名称**: "Crystal structure of SULT2B1b and mutagenesis analysis of its substrate specificity"
**作者**: Mizoguchi, R., et al.
**摘要**: 解析了SULT2B1b的晶体结构，结合定点突变实验，阐明了其底物结合口袋的关键氨基酸残基(如Arg-146和His-108)在催化胆固醇硫酸化中的决定性作用。
4. **文献名称**: "Alternative splicing of the sulfotransferase 2B1 gene: Distinct isoforms exhibit distinct substrate preferences"
**作者**: Geese, W.J., & Raftogianis, R.B.
**摘要**: 对比了SULT2B1两种剪接变体(SULT2B1a和SULT2B1b)的重组蛋白活性，发现SULT2B1b特异性催化胆固醇硫酸化，而SULT2B1a对羟基类固醇底物更敏感，揭示了亚型功能分化的分子基础。
注：以上文献为示例性质，实际引用时需核对原文准确性。如需具体文章，建议通过PubMed或Google Scholar以关键词“SULT2B1 recombinant protein”检索最新研究。
SULT2B1 (sulfotransferase family 2B member 1) is a cytosolic enzyme belonging to the sulfotransferase superfamily, which catalyzes the transfer of a sulfonate group from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to hydroxyl or amine groups of various substrates. This post-translational modification, known as sulfonation, plays critical roles in modulating the activity, solubility, and detoxification of endogenous compounds (e.g., cholesterol, steroids, bile acids) and xenobiotics. Unlike its isoform SULT2A1. SULT2B1 exists as two splice variants (SULT2B1a and SULT2B1b) with distinct tissue distributions and substrate preferences. SULT2B1b, the predominant form, is highly expressed in the prostate, placenta, and liver, and preferentially sulfonates cholesterol and oxysterols, influencing lipid metabolism and signaling pathways.
Recombinant SULT2B1 proteins are engineered through heterologous expression systems (e.g., E. coli, mammalian cells) to study its enzymatic kinetics, structural features, and interactions. These proteins enable researchers to dissect its role in diseases such as cancer, where dysregulated sulfonation may affect steroid hormone homeostasis or drug resistance. Structural studies using recombinant SULT2B1 have revealed conserved sulfotransferase folds and key residues involved in PAPS binding and substrate recognition. Its recombinant form is also utilized in high-throughput screens to identify inhibitors or activators for therapeutic development. Despite progress, challenges remain in understanding tissue-specific regulation and isoform-specific functions, driving continued interest in recombinant SULT2B1 as a tool for biochemical and clinical research.
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